Creation of a highly stable direct electron transfer-type enzyme sensor by combining a hyperthermophilic dehydrogenase and natural electron mediator.

Miku Maeno, Haruhiko Sakuraba, Toshihisa Ohshima, Shin-Ichiro Suye, Takenori Satomura

Abstract read

In one paragraph

Article in Biotechnology letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Miku MaenoAdvanced Interdisciplinary Science and Technology, Graduate School of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui, 910-8507, Japan.

Haruhiko SakurabaDepartment of Applied Biological Science, Faculty of Agriculture, Kagawa University, Kagawa, 761-0795, Japan.

Toshihisa OhshimaDepartment of Biomedical Engineering, Osaka Institute of Technology, Osaka, 535-8585, Japan.

Shin-Ichiro SuyeDivision of Engineering, Faculty of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui, 910-8507, Japan.

Takenori SatomuraDivision of Engineering, Faculty of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui, 910-8507, Japan. satomura@u-fukui.ac.jp.ORCID http://orcid.org/0000-0003-0151-9691

Funding

Japan Society for the Promotion of Science KAKENHI grants 24K08707JST SPRING JPMJSP2176

6 · The paper itself

Abstract

This study aimed to address the stability limitations of third-generation biosensors using enzymes from mesophilic organisms, by engineering a stable direct electron transfer (DET)-type dehydrogenase capable of transferring electrons extracted from the substrate to the electrode. A fusion protein combining the mediated electron transfer (MET)-type aldose sugar dehydrogenase from the hyperthermophile Pyrobaculum aerophilum (PaeASD), which cannot transfer electrons generated by enzymatic reactions to the electrode without a mediator, and the natural electron transfer protein cytochrome b

Indexed as

Biosensing TechniquesCarbohydrate DehydrogenasesPyrobaculumCytochrome b GroupElectron TransportEnzyme StabilityEscherichia coliGlucosePQQ CofactorRecombinant Fusion ProteinsCarbohydrate DehydrogenasesCytochrome b GroupGlucosePQQ CofactorRecombinant Fusion ProteinsBiosensorDirect electron transferFusion proteinMediated electron transfer

Identifiers

PMID40259038

DOI10.1093/protein/12.1.63

PMCPMC12011955

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.