ArticleScientific reports2025
LncRNA NEAT1 sponges miR-214-3p to promote osteoblast differentiation through regulating the PI3K/AKT/mTOR pathway in aortic valve calcification.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Long non-coding RNAs: Emerging regulators of diverse programmed cell death pathways in neurons.Neural regeneration research · 2026Article
- MIR31HG Modulates IL-4 Secretion by Keratinocytes and M2-Like Macrophage Polarization to Promote Local Immunosuppression in Condyloma Acuminata.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2026Article
- miR-181d-5p promotes valvular calcification and directly targets Wnt inhibitory factor 1 in calcific aortic valve disease.Molecular and cellular biochemistry · 2026Article
- LncRNA SNHG14 drives alveolar inflammation in bronchopulmonary dysplasia via DNMT3a-mediated epigenetic silencing of miR-214-3p.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2026Article
- Review
- Regulatory Roles of Long Non-Coding RNAs in Methotrexate Pharmacology: Mechanistic and Translational Insights.Pharmaceutical research · 2026Review
- Whole-transcriptome analysis of Aortic Stenosis reveals dysregulated RNA networks, immune cell infiltration, and NADK2 as a candidate regulator.Hereditas · 2026Article
- Integrated bioinformatic analysis and experiments reveal EFEMP1 as a novel aging-related signature gene in calcific aortic valve disease.Scientific reports · 2026Article
- Serotonin transporter downregulation is associated with aortic stenosis, and early profibrotic remodeling is mitigated by pharmacological inhibition of HTR2B receptor.Frontiers in cardiovascular medicine · 2026Article
- The silent dialogue between cells: regulatory roles of non-coding RNAs in cardiovascular disease.Frontiers in cell and developmental biology · 2026Review
- Long non-coding RNAs as molecular hubs integrating inflammatory and osteogenic pathways in calcific aortic valve disease.Frontiers in cardiovascular medicine · 2026Review
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Authors and funding
4 authors.
Funding
Abstract
Calcific aortic valve disease (CAVD) is the common valvular disease associated with significant morbidity and mortality. Dysregulation of long non-coding RNA (lncRNA) has been implicated in the pathogenesis of CAVD. This study aims to investigate the role of NEAT1 in CAVD pathogenesis. NEAT1, miR-214-3p and mRNA expressions were determined by qRT-PCR. Protein expressions were detected by Western blotting. Mineralized bone matrix formation was assessed by Alizarin Red staining. The osteogenic phenotype was evaluated by the alkaline phosphatase activity assay. Dual-luciferase assays were employed to confirm the binding interactions between NEAT1 and miR-214-3p, miR-214-3p and PTEN. NEAT1 was up-regulated in calcific aortic valves and after osteogenic induction of valve interstitial cells (VICs). NEAT1 could act as a positive regulator of osteogenic differentiation by repressing miR-214-3p and thereby promote expression of osteoblast-specific markers. Mechanistically, we identified PTEN as a direct target of miR-214-3p. PTEN could regulate the PI3K/AKT/mTOR pathway and participate in osteogenic differentiation. Importantly, NEAT1 could directly interact with miR-214-3p and change of miR-214-3p expression could efficiently reverse PTEN expression and osteogenic differentiation induced by NEAT1. Thus, NEAT1 positively regulated PTEN expression and activated autophagy through sponging miR-214-3p, and promoted osteogenic differentiation through the PI3K/AKT/mTOR pathway. In conclusion, we elucidates the vital function of NEAT1 as a miRNA sponge in CAVD pathogenesis, and sheds new light on lncRNA-directed diagnostic and therapeutic strategies for CAVD.
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