Evidence map›Paper›PMID 40258782›Full record

ArticleJournal of cachexia, sarcopenia and muscle2025

Oestrogen Receptor Alpha in Myocyte Maintains Muscle Regeneration in Duchenne Muscular Dystrophy.

Xiaofei Huang, Sijia Li, Huna Wang, Lei Zhao, Xihua Li, Shusheng Fan, Wanting Hu, Haowei Tong, Guangyao Guo, Dengqiu Xu and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cachexia, sarcopenia and muscle, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Xiaofei HuangNew Drug Screening and Pharmacodynamics Evaluation Center, China Pharmaceutical University, Nanjing, China.
Sijia LiNew Drug Screening and Pharmacodynamics Evaluation Center, China Pharmaceutical University, Nanjing, China.
Huna WangNew Drug Screening and Pharmacodynamics Evaluation Center, China Pharmaceutical University, Nanjing, China.
Lei ZhaoDepartment of Neurology Children's, Hospital of Fudan University, Shanghai, China.
Xihua LiDepartment of Neurology Children's, Hospital of Fudan University, Shanghai, China.
Shusheng FanNew Drug Screening and Pharmacodynamics Evaluation Center, China Pharmaceutical University, Nanjing, China.
Wanting HuNew Drug Screening and Pharmacodynamics Evaluation Center, China Pharmaceutical University, Nanjing, China.
Haowei TongNew Drug Screening and Pharmacodynamics Evaluation Center, China Pharmaceutical University, Nanjing, China.
Guangyao GuoNew Drug Screening and Pharmacodynamics Evaluation Center, China Pharmaceutical University, Nanjing, China.
Dengqiu XuDepartment of Hepatobiliary Surgery Innovative Institute of Tumor Immunity and Medicine (ITIM), Anhui Province key Laboratory of Tumor Immune Microenvironment and Immunotherapy, The First Affiliated Hospital of Anhui Medical University, Hefei, China.
Luyong ZhangNew Drug Screening and Pharmacodynamics Evaluation Center, China Pharmaceutical University, Nanjing, China.
Zhenzhou JiangNew Drug Screening and Pharmacodynamics Evaluation Center, China Pharmaceutical University, Nanjing, China.ORCID 0000-0002-0420-0822
Qinwei YuNew Drug Screening and Pharmacodynamics Evaluation Center, China Pharmaceutical University, Nanjing, China.

Funding

China Postdoctoral Science Foundation 2020M681786'Double First-Class' University project CPU2018GY33National Key Research and Development Program of China 2022YFC2703600National Natural Science Foundation of China 81773827National Natural Science Foundation of China 82304642Natural Science Foundation of Jiangsu Province BK20210430
6 · The paper itself

Abstract

backgroundOestrogen receptor alpha (ERα) plays an important role in maintaining mitochondrial function and regulating metabolism in skeletal muscle. However, its alterations and potential mechanisms in Duchenne muscular dystrophy (DMD) remain incompletely understood. In this study, we demonstrated the protective role of ERα in myocyte for skeletal muscle regeneration in mdx mice and explored the therapeutic effects of oestrogen receptor modulators on DMD.

methodsDMD patients' biopsies were obtained for histological analysis to explore the expression of ERα. The phenotype of muscle was analysed by histology and molecular biology. The therapeutical effect of different oestrogen receptor modulators was examined in mdx mice treated with fulvestrant (FVT, 20 mg/kg once a week) or oestradiol (E2, 1 mg/kg per day) for 4 weeks. The protective effect of ERα was performed on mdx mice after conditional knockout of ERα in skeletal muscle (ERα

resultsThe ERα expression was increased in DMD patients' triceps (p < 0.05) and mdx mice muscles (p < 0.05). FVT reduced ERα levels in the mdx mice muscles (p < 0.01) but had no significant effect on skeletal muscle regeneration on mdx mice. Compared with mdx mice, E2 reduced the levels of creatine kinase (CK) and lactic dehydrogenase (LDH) (p < 0.001) in serum, enhanced skeletal muscle function, alleviated skeletal muscle atrophy and fibre loss and upregulated the expression of ERα in GAS (p < 0.001) and TA (p < 0.05). The myogenic factors such as myosin heavy chain (MyHC, p < 0.001), myogenin (MyoG, p < 0.05), MyoD (p < 0.05) and ERRα (p < 0.001) were increased in mdx mice GAS with E2. But E2 had no effect on ERα

conclusionWe demonstrated that ERα in myocyte exerted a protective effect on skeletal muscle regeneration in DMD patients and mdx mice through the ERα-ERRα-MyoD pathway, which has potential implications for DMD therapy strategies.

Indexed as

Estrogen Receptor alphaMuscle, SkeletalMuscular Dystrophy, DuchenneRegenerationAnimalsDisease Models, AnimalFemaleHumansMaleMiceMice, Inbred mdxEstrogen Receptor alphaDuchenne muscular dystrophyfulvestrantoestradioloestrogen receptor alphaskeletal muscle regeneration

Identifiers

PMID40258782
PMCPMC12011492

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.