Evidence map›Paper›PMID 40257620›Full record

ArticlePlant cell reports2025

RsWRKY44 participated in anthocyanin biosynthesis regulation in radish through interaction with RsMYB1a.

Biao Lai, Chenxi Gao, Li Jiang, Li Wen, Xushuo Zhang, Wei Shen, Yanling Yu, Hanbing Yang, Fabo Chen, Ping Fang and 1 more

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Article in Plant cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Biao LaiSchool of Advanced Agriculture and Bioengineering, Yangtze Normal University, 408100, Chongqing, People's Republic of China.
Chenxi GaoSchool of Advanced Agriculture and Bioengineering, Yangtze Normal University, 408100, Chongqing, People's Republic of China.
Li JiangSchool of Advanced Agriculture and Bioengineering, Yangtze Normal University, 408100, Chongqing, People's Republic of China.
Li WenSchool of Advanced Agriculture and Bioengineering, Yangtze Normal University, 408100, Chongqing, People's Republic of China.
Xushuo ZhangSchool of Advanced Agriculture and Bioengineering, Yangtze Normal University, 408100, Chongqing, People's Republic of China.
Wei ShenSchool of Advanced Agriculture and Bioengineering, Yangtze Normal University, 408100, Chongqing, People's Republic of China.
Yanling YuSchool of Advanced Agriculture and Bioengineering, Yangtze Normal University, 408100, Chongqing, People's Republic of China.
Hanbing YangSchool of Advanced Agriculture and Bioengineering, Yangtze Normal University, 408100, Chongqing, People's Republic of China.
Fabo ChenSchool of Advanced Agriculture and Bioengineering, Yangtze Normal University, 408100, Chongqing, People's Republic of China.
Ping FangSchool of Advanced Agriculture and Bioengineering, Yangtze Normal University, 408100, Chongqing, People's Republic of China.
Lina DuSchool of Advanced Agriculture and Bioengineering, Yangtze Normal University, 408100, Chongqing, People's Republic of China. dulina05@163.com.ORCID http://orcid.org/0000-0002-1504-8571

Funding

Chongqing Natural Science Foundation CSTB2022NSCQ-MSX0228Natural Science Foundation of Chongqing Municipality CSTC2021JCYJ-MSXMX0083Yangtze Normal University 2016KYQD20Yangtze Normal University 2016XJQN06
6 · The paper itself

Abstract

key messageRsWRKY44 transcription factor, associated with anthocyanin biosynthesis in different radish cultivars, highly facilitates the activation of RsCHI and RsUFGT genes through its interaction with RsMYB1a, thereby promoting anthocyanin production. The regulation of anthocyanin biosynthesis in radish is primarily controlled by RsMYB1a and RsbHLH4, while the involvement of other factors in this process is not well understood. This study identified a WRKY transcription factor, RsWRKY44, as a key player in anthocyanin biosynthesis regulation. The expression of RsWRKY44 showed a strong correlation with anthocyanin content across different radish cultivars. RsWRKY44 was found to be expressed in the nuclei and exhibit transactivation activity. It was observed that only when RsWRKY44 was co-expressed with RsMYB1a, anthocyanin accumulation was induced in tobacco leaves, while RsWRKY44 alone did not. Additionally, RsWRKY44, along with RsMYB1a, activated the expression of tobacco endogenous anthocyanin biosynthesis regulatory genes NtAN1a and NtAN1b, as well as the structural genes NtCHS, NtCHI, NtDFR, NtF3H, NtANS, NtUFGT in transgenic tobacco. BiFC, FLC, and DLA assays confirmed the interaction between RsWRKY44 and RsMYB1a leading to the activation of radish genes RsCHI and RsUFGT, promoting anthocyanin biosynthesis. This study sheds light on a new molecular mechanism of RsWRKY44 involved in anthocyanin biosynthesis regulation in radish.

Indexed as

AnthocyaninsPlant ProteinsRaphanusTranscription FactorsGene Expression Regulation, PlantNicotianaPlant LeavesPlants, Genetically ModifiedAnthocyaninsPlant ProteinsTranscription FactorsAnthocyaninRadishRsMYB1aRsWRKY44

Identifiers

PMID40257620

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