ArticleMethods in molecular biology (Clifton, N.J.)2025
RNA Immunoprecipitation (RIP) from Purified Nuclei in Cells.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
1 citing paper in PubMed.
- MeCP2 NID interaction with RNA: implications for Rett syndrome-relevant protein regulation.Human molecular genetics · 2026Article
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Authors and funding
2 authors.
Funding
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Abstract
Identifying and assaying protein-RNA interactions is foundational to understanding the molecules' role in both the cell and organism as a whole. Importantly, functional noncoding RNAs and their protein partners have presented RNA researchers with a new vast list of these interactions, which often do not occur through the well-described, or, canonical mechanisms, opening a floodgate of research potential for years to come. With this in mind, it is necessary to standardize assay methods, with good understanding of points of optimization. Here, we describe a simple protocol for RNA immunoprecipitation (RIP) from purified nuclei of cells. Purification of nuclei prior to RIP is important to eliminate false-positive nuclear protein-RNA interactions, especially given that specific binding to ncRNA seems to be based on cumulative electrostatic forces rather than lock-and-key binding.
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Registered trials
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