ArticleMethods in molecular biology (Clifton, N.J.)2025
Efficient In-house Coupling of Sample and Library Preparation for ChIP-Seq of Histone Modifications in Complex Plant Tissues.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
ChIP-seq is a commonly used method to characterize chromatin-associated features on a genome-wide basis. A common set of steps for a ChIP-seq procedure prior to sequencing includes crosslinking, nuclei extraction, chromatin shearing, immunoprecipitation, elution, reversal of crosslinks, and library preparation. Plant material can be challenging to process for ChIP-seq analysis due to the unique attributes of plant cells that impair success. Here, we describe an effective ChIP-seq sample preparation method that is optimized for generating robust libraries from complex plant materials. In particular, we identify time as a critical parameter for effective coupling of ChIP-seq sample preparation with a commercially available kit to generate robust NGS libraries in-house. The resulting protocol is a cost-effective strategy to generate reliable ChIP-seq libraries from complex plant material and thereby acquire representative sequencing data.
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