Evidence map›Paper›PMID 40257562›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Genome-Wide Nucleosome Mapping by H3Q85C-Directed Chemical Cleavage in Saccharomyces cerevisiae.

Célia Jeronimo, Christian Poitras, François Robert

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Célia JeronimoInstitut de recherches cliniques de Montréal, Montréal, Québec, Canada.
Christian PoitrasInstitut de recherches cliniques de Montréal, Montréal, Québec, Canada.
François RobertInstitut de recherches cliniques de Montréal, Montréal, Québec, Canada. francois.robert@ircm.qc.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mapping the position of nucleosomes in vivo is key to our understanding of chromatin-based processes such as gene transcription, DNA replication, and repair. Methods based on micrococcal nuclease (MNase) digestion of chromatin are widely used to map nucleosomes, but these methods suffer from some limitations. More recently, nucleosome mapping methods that take advantage of our ability to convert cysteine residues-carefully inserted in histone proteins-into nucleases provide alternatives to MNase-based assays. Here, we provide a detailed protocol for the mapping of nucleosomes via H3Q85C-directed DNA cleavage in Saccharomyces cerevisiae.

Indexed as

Chromosome MappingGenome, FungalHistonesNucleosomesSaccharomyces cerevisiaeDNA CleavageDNA, FungalMicrococcal NucleaseSaccharomyces cerevisiae ProteinsDNA, FungalHistonesMicrococcal NucleaseNucleosomesSaccharomyces cerevisiae ProteinsBase-pair resolutionChemical cleavageHistone H3HistoneH3 mutant Q85CNucleosomal linker sizeNucleosome mappingNucleosome repeat lengthS. cerevisiae

Identifiers

PMID40257562

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.