Evidence map›Paper›PMID 40257401›Full record

ArticleThe Journal of cell biology2025

NEMF-mediated CAT tailing facilitates translocation-associated quality control.

Amanda Ennis, Lihui Wang, Yue Xu, Layla Saidi, Xiaorong Wang, Clinton Yu, Sijung Yun, Lan Huang, Yihong Ye

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of cell biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Amanda Ennis *Laboratory of Molecular Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.ORCID 0009-0003-8694-022X
Lihui Wang *Laboratory of Molecular Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.ORCID 0009-0000-2896-9380
Yue XuLaboratory of Molecular Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.ORCID 0000-0002-0958-8846
Layla SaidiLaboratory of Molecular Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.ORCID 0009-0001-2768-0988
Xiaorong WangDepartment of Physiology and Biophysics, University of California Irvine, Irvine, CA, USA.ORCID 0009-0007-6700-1277
Clinton YuDepartment of Physiology and Biophysics, University of California Irvine, Irvine, CA, USA.ORCID 0000-0002-2931-5474
Sijung YunLaboratory of Molecular Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.ORCID 0000-0003-1948-3690
Lan HuangDepartment of Physiology and Biophysics, University of California Irvine, Irvine, CA, USA.ORCID 0000-0002-3140-4687
Yihong YeLaboratory of Molecular Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.ORCID 0000-0002-9512-7922

Funding

Supplement: Advancing Proteomics Technologies to Decipher the Ubiquitin-Proteasome SystemR35GM145249 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI Lan Huang · 2022 to 2026
$3.2M
NIDDK NIH HHSNIGMS NIH HHS R35 GM145249NIH HHS R35GM145249
6 · The paper itself

Abstract

Ribosome stalling during co-translational translocation at the ER causes translocon clogging and impairs ER protein biogenesis. Mammalian cells resolve translocon clogging via a poorly characterized translocation-associated quality control (TAQC) process. Here, we combine a genome-wide CRISPR screen with live-cell imaging to dissect the molecular linchpin of TAQC. We show that TAQC substrates translated from mRNAs bearing a ribosome-stalling poly(A) sequence are degraded by lysosomes and the proteasome. By contrast, the degradation of defective nascent chains encoded by nonstop (NS) mRNAs involves an unconventional ER-associated protein degradation (ERAD) mechanism depending on ER-to-Golgi trafficking, KDEL-mediated substrate retrieval at the Golgi, and a tRNA-binding factor NEMF that appends an aggregation-prone carboxyl tail to stalled NS nascent chains. We propose that NEMF-mediated CAT tailing targets a subset of TAQC substrates via Golgi retrieval for ERAD, safeguarding ER homeostasis.

Indexed as

Endoplasmic Reticulum-Associated DegradationAnimalsEndoplasmic ReticulumGolgi ApparatusHEK293 CellsHeLa CellsHumansLysosomesProteasome Endopeptidase ComplexProtein BiosynthesisProtein TransportRibosomesRNA, MessengerProteasome Endopeptidase ComplexRNA, Messenger

Identifiers

PMID40257401
PMCPMC12010919

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.