Overexpression, Purification, and Biochemical Characterization of the vanC2 d-Ala-d-Ser Ligase from

Sneha B Paymal, Sagar S Barale, Shirishkumar V Supanekar, Kailas D Sonawane, Kiran D Pawar

Abstract read

In one paragraph

Article in ACS omega, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Sneha B PaymalDepartment of Microbiology, Shivaji University, Vidyanagar, Kolhapur, Maharashtra 416004, India.ORCID https://orcid.org/0000-0002-3688-0850

Sagar S BaraleDepartment of Microbiology, School of Life Sciences, Central University of Rajasthan, Ajmer, Rajasthan 305817, India.ORCID https://orcid.org/0000-0001-5820-2412

Shirishkumar V SupanekarRayat Institute of Research and Development (RIRD), Satara, Maharashtra 415001, India.

Kailas D SonawaneDepartment of Biochemistry, Shivaji University, Vidyanagar, Kolhapur, Maharashtra 416004, India.ORCID https://orcid.org/0000-0003-0156-7466

Kiran D PawarSchool of Nanoscience and Biotechnology, Shivaji University, Vidyanagar, Kolhapur, Maharashtra 416004, India.ORCID https://orcid.org/0000-0002-2519-2454

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The bacterial cell wall and enzymes involved in peptidoglycan biosynthesis are prime targets for the discovery of novel antibacterial agents. Among these enzymes, d-alanine-d-alanine ligases (Ddl) are particularly significant due to their utilization of specific substrates (d-amino acids) essential for bacterial viability. Isozymes of Ddl that utilize alternative substrates such as d-lactate or d-serine are found in vancomycin-resistant Gram-positive bacteria, initially identified in Enterococcus species, and now represent a growing concern in clinical settings. In this study, we isolated and identified vancomycin-resistant

Identifiers

PMID40256530

DOI10.1021/acsomega.5c00599

PMCPMC12004154

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.