Evidence map›Paper›PMID 40255549›Full record

ArticleFood science & nutrition2025

Extraction and Analysis of Natural Color From Beetroot (

Salman Khalil, Salah Laaraj, Nida Firdous, Umar Farooq, Mohamed Bouhrim, Rashed N Herqash, Abdelaaty A Shahat, Ashiq Hussain, Aziz Mouhaddach, Bruno Eto and 5 more

Abstract read
In one paragraph

Article in Food science & nutrition, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Multianalytical Characterization of Elderberry (Foods (Basel, Switzerland) · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Salman KhalilFaculty of Food and Home Sciences Muhmmad Nawaz Shareef University of Agriculture Multan Pakistan.
Salah LaarajAgri-Food Technology and Quality Laboratory, Regional Centre of Agricultural Research of Tadla National Institute of Agricultural Research (INRA) Rabat Morocco.
Nida FirdousFaculty of Food and Home Sciences Muhmmad Nawaz Shareef University of Agriculture Multan Pakistan.
Umar FarooqFaculty of Food and Home Sciences Muhmmad Nawaz Shareef University of Agriculture Multan Pakistan.
Mohamed BouhrimBiological Engineering Laboratory, Faculty of Sciences and Techniques Sultan Moulay Slimane University Beni Mellal Morocco.ORCID https://orcid.org/0000-0001-9944-811X
Rashed N HerqashDepartment of Pharmacognosy College of Pharmacy, King Saud University Riyadh Saudi Arabia.
Abdelaaty A ShahatDepartment of Pharmacognosy College of Pharmacy, King Saud University Riyadh Saudi Arabia.
Ashiq HussainInstitute of Food Science and Nutrition, University of Sargodha Sargodha Pakistan.ORCID https://orcid.org/0000-0002-5239-4641
Aziz MouhaddachVegetable and Microbial Biotechnology, Biodiversity and Environment Faculty of Sciences, Mohammed V University in Rabat Rabat Morocco.
Bruno EtoLaboratoires TBC, Laboratory of Pharmacology, Pharmacokinetics, and Clinical Pharmacy Faculty of Pharmaceutical and Biological Sciences Lille France.
Aliza BatoolFaculty of Food and Home Sciences Muhmmad Nawaz Shareef University of Agriculture Multan Pakistan.
Barira BibiUniversity Institute of Biochemistry and Biotechnology PMAS Arid Agriculture University Rawalpindi Rawalpindi Pakistan.
Amina AyeshaUniversity Institute of Biochemistry and Biotechnology PMAS Arid Agriculture University Rawalpindi Rawalpindi Pakistan.
Farooq ArshadInstitute of Food Science and Nutrition, University of Sargodha Sargodha Pakistan.
Kaoutar ElfazaziAgri-Food Technology and Quality Laboratory, Regional Centre of Agricultural Research of Tadla National Institute of Agricultural Research (INRA) Rabat Morocco.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A rich source of natural color, beetroot (Beta vulgaris L. subsp. vulgaris) extracts and colorants offer deep red color to the dishes and boost their nutritional and antioxidant value. This research extracted beetroot color using Soxhlet, cold press, and ultrasonic methods. After testing the extracts for yield %, color (L*, a*, and b*), betalains, total polyphenols, and antioxidant activity, the colorant was used to make ice cream. The results of three techniques revealed that the extract obtained from Soxhlet extraction (SE) has more total phenolic content (244.11 mg GAE/100 g), DPPH free radical scavenging activity (23.41%), significantly higher yield (48.05%), and better color results, as compared to the other extraction techniques. However, the contents of betalains (399.47 mg/L) were observed more in the extract obtained from ultrasonic extraction (UE). The extract obtained after the SE technique was further utilized in the ice cream manufacturing to check its acceptability in this product, compared to the artificial color. Six samples of ice cream were developed having different ratios of beetroot-derived color and artificial color. Control ice cream (T0) contained artificial color, whereas ice cream (T5) was developed by replacing complete artificial color with beetroot-derived color. The other treatments (T1), (T2), (T3), and (T4) contained a combination of both natural and artificial colors with different proportions. Various quality parameters of these ice cream formulations, such as pH, acidity, brix, melting rate, overrun, and specific gravity, were studied. The result signified that the addition of beetroot color did not have a considerable effect on these attributes. The result of color analysis of ice cream suggested that the mean value of L* is higher in T5 (73.16), which only contains 0.1% beetroot color, and is lowest in T0 (65.24), which contains 0.1% artificial color. The addition of natural colorant resulted in a higher L* value. Sensory characteristics including color, mouth coating, flavor, aroma, texture, and overall acceptability of T5 showed more acceptance and significant results as compared to other treatments that contained artificial colors. Therefore, the beetroot-derived natural colorants could be employed to develop nutritional, healthy, and acceptable ice cream.

Indexed as

antioxidant activityBeta vulgaris Lcold press extractionice creamnatural colorantSoxhlet extractionultrasonic extraction

Identifiers

PMID40255549
PMCPMC12006923

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.