ArticleTropical animal health and production2025
Metarhizium anisopliae and diatomaceous earth for the control of Rhipicephalus microplus ticks: laboratory and field trials.
Article in Tropical animal health and production, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Advances in the use of entomopathogenic fungi to control ixodid ticks.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2026Review
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this research was to assess the efficacy of Metarhizium anisopliae (Ma) and diatomaceous earth (De), both individually and in combination, for the control of Rhipicephalus microplus ticks under laboratory and field conditions. In vitro experiments involved testing four concentrations of Ma (25%, 50%, 75%, and 100%), four concentrations of De (25%, 50%, 75%, and 100%), and three combination treatments (Ma25De75, Ma50De50, Ma75De25), alongside a control treatment of sterile distilled water with 0.1% (v/v) Tween 80, to evaluate their impact on R. microplus larvae. Laboratory trial results demonstrated that the application of Ma at 25% and 50%, as well as De at all concentrations, and the three combination treatments, led to a larval mortality exceeding 97% over a seven-day period. In contrast, individual treatments with Ma100 and Ma75 resulted in approximately 50% larval mortality. Subsequent field evaluations focused on Ma25De75 and Ma50De50 treatments for adult tick control. The application of Ma25De75 and Ma50De50 exhibited progressive increases in efficacy, reaching 97% and 88%, respectively, 21 days post-application. After the second application, efficacy further improved to 100% and 94.8% for Ma25De75 and Ma50De50, respectively. In conclusion, Ma25 and Ma50, along with all concentrations of De, proved to be effective options for controlling R. microplus larvae. Furthermore, a potential synergistic effect between M. anisopliae conidia and De was observed, demonstrating high efficacy rates of 100% in vitro for larvae and 88% to 100% in the field for adult R. microplus tick control.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.