Evidence map›Paper›PMID 40254605›Full record

ArticleNPJ vaccines2025

Gut microbiota modulate immune responses to orally and parenterally administered rotavirus in mice.

Zhenda Shi, Sung-Sil Moon, Jun Zou, Yanling Wang, Noopur Bhatnagar, Vu L Ngo, Xiaoqian Wang, Houping Wang, Theresa K Bessey, Jennifer J Hull and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in NPJ vaccines, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Zhenda ShiDivision of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. pst2@cdc.gov.
Sung-Sil MoonDivision of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.ORCID http://orcid.org/0000-0003-2553-3404
Jun ZouInstitute for Biomedical Sciences, Georgia State University, Atlanta, GA, USA.
Yanling WangInstitute for Biomedical Sciences, Georgia State University, Atlanta, GA, USA.
Noopur BhatnagarInstitute for Biomedical Sciences, Georgia State University, Atlanta, GA, USA.ORCID http://orcid.org/0000-0001-9260-3957
Vu L NgoInstitute for Biomedical Sciences, Georgia State University, Atlanta, GA, USA.
Xiaoqian WangDivision of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Houping WangDivision of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.ORCID http://orcid.org/0000-0001-7784-3485
Theresa K BesseyDivision of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Jennifer J HullDivision of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Yuhuan WangDivision of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.ORCID http://orcid.org/0000-0001-6861-6205
Sang-Moo KangInstitute for Biomedical Sciences, Georgia State University, Atlanta, GA, USA.ORCID http://orcid.org/0000-0001-6198-331X
Andrew T GewirtzInstitute for Biomedical Sciences, Georgia State University, Atlanta, GA, USA.
Baoming JiangDivision of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. bxj4@cdc.gov.ORCID http://orcid.org/0000-0003-2072-0729

Funding

DECONSTRUCTING INFLAMMATION AND ALTERED MICROBIOTA IN METABOLIC SYNDROMER01DK099071 · NIDDK · GEORGIA STATE UNIVERSITY · PI GEWIRTZ, ANDREW T · 2013 to 2024
$4.8M
Intestinal microbiota-mediated rotavirus vaccine failureR01AI170014 · NIAID · GEORGIA STATE UNIVERSITY · PI Andrew T Gewirtz, Sasirekha Ramani · 2022 to 2026
$3.9M
NIAID NIH HHS R01 AI170014NIDDK NIH HHS R01 DK099071U.S. Department of Health & Human Services | National Institutes of Health (NIH) DK099071
6 · The paper itself

Abstract

Rotavirus (RV) remains a significant cause of infantile morbidity and mortality, while oral RV vaccines offer inconsistent protection. This study investigates whether gut microbiota influence immune responses to orally and intramuscularly (IM) administered RV strains. Using murine models, we identified microbiota constituents, including segmented filamentous bacteria, reducing oral RV infection and RV antibody generation. Such blockade of RV-induced responses was associated with elevated expression of intestinal Reg3β and Reg3γ and was recapitulated by intraperitoneal administration of cognate recombinant proteins. IM administration following oral RV inoculations enhanced antibody production and defense against RV challenge. We further showed microbiota composition also influenced the efficacy of a single IM RV inoculation. Antibiotic-induced microbiota depletion boosted IM RV efficacy in poorly responding animals. Such enhancement of IM RV-induced immunity appeared to be associated with increased expression of serum RANTES and Eotaxin. The phenotype was recapitulated by directly adjuvating these chemokines to the IM inoculum.

Identifiers

PMID40254605
PMCPMC12009995

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.