ArticleMicrobial cell factories2025
Optimization of thermostable amylolytic enzyme production from Bacillus cereus isolated from a recreational warm spring via Box Behnken design and response surface methodology.
Article in Microbial cell factories, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed.
- Overcoming cellular secretion bottlenecks: advanced secretion engineering and molecular tailoring for next-generation microbial α-amylases with enhanced industrial performance.World journal of microbiology & biotechnology · 2026Review
- Towards a Novel Biocontrol Strategy: High Performance of Optimised Cell Wall-Degrading Enzymes Secreted by Escovopsis primorosea LBM 277.Environmental microbiology reports · 2026Article
- Harnessing Bacillus velezensis for enhanced protease biosynthesis: optimization, purification, and kinetic profiling.BMC microbiology · 2026Article
- Simplified enzyme activity-based screening of microorganisms from Korean fermented foods for food-waste decomposition.Biodegradation · 2025Article
- Green starch nanoparticles production in situ using α-amylase from a newly isolated Bacillus subtilis strain-MA6: statistical designs and characterizations.Microbial cell factories · 2025Article
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Authors and funding
4 authors.
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Abstract
This study aimed to find a source for local amylase-producing microbes. Sixteen isolates were obtained from the water samples from the warm spring and characterized based on morphological and biochemical tests. The 16S rRNA molecular identification technique confirmed the most potent isolate as Bacillus cereus. The thermophilic property of the bacterium demonstrated that it could withstand temperatures of up to 80 °C. One-factor-at-a-time (OFAT) and Box Behnken Design (BBD) coupled with response surface methodology (RSM) optimization techniques were used to improve amylase production. OFAT established optimal physical parameter conditions as the starch concentration of 5% w/v, inoculum volume of 2% v/v, pH of 8, incubation temperature of 45 °C, and 48 h of incubation, leading to amylase activity of 172.6 U/mL by the isolated B. cereus. A quadratic mathematical model with a coefficient of determination (R
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