Evidence map›Paper›PMID 40252130›Full record

ArticleCancer immunology, immunotherapy : CII2025

Identification and validation of poor prognosis immunoevasive subtype of esophageal cancer with tumor-infiltrating SAMD3 + NK cell abundance.

Xu Huang, Runze You, Fangyi Liu, Zitao Jian, Guanyou Zhou, Hao Yin, Mengyuan Wu, Tiantao Sun, Zhiyun Duan, Wenyi Xu and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer immunology, immunotherapy : CII, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Xu Huang *Departments of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Runze You *Departments of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Fangyi Liu *The School of Basic Medical Sciences, Fudan University, Shanghai, China.
Zitao Jian *Departments of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Guanyou ZhouThe School of Basic Medical Sciences, Fudan University, Shanghai, China.
Hao YinDepartments of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Mengyuan WuDepartments of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Tiantao SunDepartments of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Zhiyun DuanDepartments of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Wenyi XuDepartments of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Shaoyuan ZhangDepartments of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Xinyu YangDepartments of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Heng JiaoDepartments of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Shuyi YangCancer Center, Zhongshan Hospital, Fudan University, Shanghai, China.
Qingle WangCancer Center, Zhongshan Hospital, Fudan University, Shanghai, China.
Jun YinDepartments of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Han TangDepartments of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Miao LinDepartments of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China. linmiao@fudan.edu.cn.
Lijie TanDepartments of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China. tan.lijie@zs-hospital.sh.cn.

Funding

Fujian Provincial Health Technology Project 2021GGB03National Natural Science Foundation of China 81902396Science and Technology Commission of Shanghai Municipality 23S31900400Zhongshan Hospital 2020ZSLC5，2021ZSYQ27
6 · The paper itself

Abstract

introductionEsophageal cancer (EC) remains highly lethal due to tumor microenvironment (TME)-mediated immune evasion. While natural killer (NK) cells are central to antitumor immunity, their functional states in EC are poorly characterized.

methodsWe integrated bulk RNA-seq (TCGA/GEO) and single-cell data to construct an NK cell-derived prognostic signature (NK score) via LASSO-Cox regression. Immunofluorescence was applied to assess the clinical relevance of SAMD3 + NK cells in EC. Using both xenograft mouse models and in vitro co-culture procedures, the impact of SAMD3 on NK cell function was confirmed.

resultsIn EC patients, the prognostic NK score-which is generated from important NK cell markers including SAMD3-was substantially correlated with a worse chance of survival. NK cells within the TME had significant levels of SAMD3 expression, as seen by immunofluorescence labeling. Moreover, NK cells with SAMD3 knockdown exhibited enhanced antitumor activity, leading to decreased tumor development in the xenograft model. DISCUSSION: Our results demonstrate the predictive significance of NK cell markers in EC and pinpoint SAMD3 as a critical modulator of NK cell activity. We pioneer SAMD3 + NK cells as architects of TME immunosuppression in EC. Our findings nominate SAMD3 inhibition as a combinatorial strategy to overcome immune checkpoint blockade resistance.

Indexed as

Biomarkers, TumorEsophageal NeoplasmsKiller Cells, NaturalLymphocytes, Tumor-InfiltratingTumor EscapeAnimalsFemaleHumansMaleMicePrognosisTumor MicroenvironmentXenograft Model Antitumor AssaysBiomarkers, TumorEsophageal cancerNatural killer cellSAMD3

Identifiers

PMID40252130
PMCPMC12009252

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.