Evidence map›Paper›PMID 40252089›Full record

ArticleCurrent microbiology2025

Genomic Characterization of Serratia fonticola (EBS19) as a Biocontrol Agent against Botrytis cinerea.

Efe Berk Bozkurt, Ömür Baysal, Anna Marzec-Grządziel, Ragıp Soner Silme, Ahmet Can, İlayda Nur Belen, Ümran Çapar, Ahmet Korkut

Abstract read
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In one paragraph

Article in Current microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Efe Berk Bozkurt *Molecular Microbiology Unit in Department of Molecular Biology and Genetics, Faculty of Science, Muğla Sıtkı Koçman University, 48121, Kötekli-Muğla, Turkey.ORCID https://orcid.org/0000-0001-9871-1713
Ömür Baysal *Molecular Microbiology Unit in Department of Molecular Biology and Genetics, Faculty of Science, Muğla Sıtkı Koçman University, 48121, Kötekli-Muğla, Turkey. omurbaysal@mu.edu.tr.ORCID http://orcid.org/0000-0001-5104-0983
Anna Marzec-GrządzielDepartment of Agricultural Microbiology, Institute of Soil Science and Plant Cultivation, Puławy, Poland.ORCID https://orcid.org/0000-0002-0589-0714
Ragıp Soner SilmeCentre for Research and Practice in Biotechnology and Genetic Engineering, Istanbul University, Fatih, 3400, Istanbul, Turkey.ORCID https://orcid.org/0000-0001-6547-3747
Ahmet CanMolecular Microbiology Unit in Department of Molecular Biology and Genetics, Faculty of Science, Muğla Sıtkı Koçman University, 48121, Kötekli-Muğla, Turkey.ORCID https://orcid.org/0000-0003-2197-0885
İlayda Nur BelenMolecular Microbiology Unit in Department of Molecular Biology and Genetics, Faculty of Science, Muğla Sıtkı Koçman University, 48121, Kötekli-Muğla, Turkey.ORCID https://orcid.org/0000-0002-5254-2931
Ümran ÇaparMolecular Microbiology Unit in Department of Molecular Biology and Genetics, Faculty of Science, Muğla Sıtkı Koçman University, 48121, Kötekli-Muğla, Turkey.ORCID https://orcid.org/0009-0005-2833-4365
Ahmet KorkutMolecular Microbiology Unit in Department of Molecular Biology and Genetics, Faculty of Science, Muğla Sıtkı Koçman University, 48121, Kötekli-Muğla, Turkey.ORCID https://orcid.org/0000-0002-4258-0627

Funding

Muğla Sıtkı Koçman Üniversitesi 19/079/12/1/2
6 · The paper itself

Abstract

Botrytis cinerea (Bc), a plant pathogenic fungus, is the causative agent of gray mold disease, which rapidly develops resistance to fungicides in cultivation areas. This study explores the biocontrol potential of various bacterial isolates collected from the rhizosphere of tomato plants (Solanum lycopersicum). Bacterial isolates were purified and neutralized through phenol vaporization for 2 days. Colonies that inhibited pathogen spore growth were confirmed via antibiosis effect using in vitro bioassays. Bacterial colonies demonstrated up to 84% inhibition of pathogen growth at 7-day post-inoculation (dpi) with a one-layer agar diffusion test and up to 70% inhibition with a double-layer agar diffusion test, compared to control plates. Both bacterial suspension and filtrate significantly suppressed pathogen mycelium growth at 11 and 14 dpi. The isolate used in further studies was identified as Serratia fonticola (EBS19) through whole-genome sequencing. Annotated genome data revealed the presence of genes encoding enzymes crucial for pathogen inhibition. Carbon preference analyses identified specific carbon sources unique to the bacterial strain. These findings are advantageous for developing effective biopreparations that ensure bacterial strain stability in practical applications. In addition, the primary focus was on the interaction between the pathogen's major stress regulator protein (BAG1) and the bacterial glycoside hydrolase. Protein-protein docking analyses elucidated strong interaction between BAG1 and bacterial glycoside hydrolase. In conclusion, this study provides a knowledge for further research using recombinant DNA and gene cloning techniques on the bacterium's mapped genome.

Indexed as

AntibiosisBiological Control AgentsBotrytisPlant DiseasesSerratiaBacterial ProteinsGenome, BacterialRhizosphereSolanum lycopersicumBacterial ProteinsBiological Control Agents

Identifiers

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.