Evidence map›Paper›PMID 40251857›Full record

ArticleThe Journal of heredity2025

The genome assembly of the duckweed fern, Azolla caroliniana.

Michael J Song, Yanã C Rizzieri, Fay-Wei Li, Forrest Freund, Merly Escalona, Erin Toffelmier, Courtney Miller, H Bradley Shaffer, Oanh Nguyen, Mohan P A Marimuthu and 5 more

Abstract read
In one paragraph

Article in The Journal of heredity, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Michael J SongSkyline College, San Bruno, CA, United States.ORCID 0000-0001-5961-753X
Yanã C RizzieriBoyce Thompson Institute, Ithaca, NY, United States.
Fay-Wei LiBoyce Thompson Institute, Ithaca, NY, United States.ORCID 0000-0002-0076-0152
Forrest FreundBureau of Land Management, United States.
Merly EscalonaDepartment of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA, United States.ORCID 0000-0003-0213-4777
Erin ToffelmierDepartment of Ecology and Evolutionary Biology, University of California, Los Angeles, Los Angeles, CA, United States.ORCID 0000-0001-6028-8497
Courtney MillerDepartment of Ecology and Evolutionary Biology, University of California, Los Angeles, Los Angeles, CA, United States.ORCID 0000-0002-4347-6629
H Bradley ShafferDepartment of Ecology and Evolutionary Biology, University of California, Los Angeles, Los Angeles, CA, United States.ORCID 0000-0002-5795-9242
Oanh NguyenDNA Technologies and Expression Analysis Core Laboratory, Genome Center, University of California, Davis, Davis, CA, United States.ORCID 0000-0003-1492-2084
Mohan P A MarimuthuDNA Technologies and Expression Analysis Core Laboratory, Genome Center, University of California, Davis, Davis, CA, United States.ORCID 0000-0001-6121-3286
Noravit ChumchimDNA Technologies and Expression Analysis Core Laboratory, Genome Center, University of California, Davis, Davis, CA, United States.ORCID 0000-0003-1139-9427
Carrie TribbleDepartment of Biology and Burke Museum of Natural History and Culture, University of Washington, Seattle, WA, United States.ORCID 0000-0001-7263-7885
Colin W FairbairnDepartment of Ecology and Evolutionary Biology, University of California, Santa Cruz, Santa Cruz, CA, United States.ORCID 0009-0007-8129-0095
William SeligmannDepartment of Ecology and Evolutionary Biology, University of California, Santa Cruz, Santa Cruz, CA, United States.ORCID 0000-0002-5762-3095
Carl J RothfelsEcology Center and Department of Biology, Utah State University, Logan, UT, United States.ORCID 0000-0002-6605-1770

Funding

Pacific Biosciences PacBio RS Single Molecule Real Time SequencerS10OD018174 · OD · UNIVERSITY OF CALIFORNIA BERKELEY · PI ROKHSAR, DANIEL SOLEYMAN · 2014 to 2014
$600k
Acquisition of Covaris E220 and Sciclone G3 systems for high throughput sequencinS10OD010786 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI COMAI, LUCA · 2012 to 2012
$311k
California Conservation Genomics Project RSI-19-690224NIH HHS S10 OD010786NIH HHS S10 OD018174
6 · The paper itself

Abstract

Azolla is a genus of freshwater ferns that is economically important as a nitrogen-fixing biofertilizer, biofuel, bioremediator, and for potential carbon sequestration, but also contains weedy invasive species. In California, only 2 species are currently recognized but the actual diversity may include up to 6 species, with the discrepancy being due to the difficulty in identifying taxa, hybridization, and the introduction of non-native species. Here, we report a new haplotype-resolved, chromosome-level assembly and annotation of Azolla caroliniana as part of the California Conservation Genomics Project (CCGP), using a combination of PacBio HiFi and Omni-C sequencing technologies. The assembly is 521 Mb in length, with a contig N50 of 1.6 Mb, and is scaffolded into 22 pseudochromosomes. A total of 21,848 protein-coding genes was predicted with a Benchmarking Universal Single-Copy Orthologs (BUSCO) completeness score of 89.88%. In combination with the previously published Azola filiculoides genome, this A. caroliniana genome will be a powerful tool for understanding the population genetics and taxonomy of one of the most cryptic, economically important, and poorly circumscribed fern taxa, and for facilitating land plant genomics more broadly.

Indexed as

FernsGenome, PlantCaliforniaChromosomes, PlantGenomicsHaplotypesMolecular Sequence AnnotationAzollaCalifornia Conservation Genomics ProjectCCGPdiploid genome assemblyHiFiOmni-Creference genome

Identifiers

PMID40251857
PMCPMC12400808

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.