ArticleMicrobial cell factories2025
Development and construction of a novel Bacillus subtilis autoinducible extracellular expression system based on a LuxI/R device.
Article in Microbial cell factories, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed.
- Psychrophilic Quorum Sensing Genes Enable Unimodal, Adjustable Protein Expression Across the EntireBiotech (Basel (Switzerland)) · 2026Article
- Engineering genetic elements for microbial protein expression systems: Advances, challenges, applications, and prospects.Synthetic and systems biotechnology · 2026Review
- Optimization Strategy of Expression Vectors and Regulatory Elements for Enhanced Protein Production inInternational journal of molecular sciences · 2025Review
- A quorum sensing-controlled type I CRISPRi toolkit for dynamically regulating metabolic flux.Nucleic acids research · 2025Article
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Abstract
backgroundMicrobial chassis expression systems are valuable tools in biotechnology and synthetic biology, and Bacillus subtilis is an important industrial microbial chassis. Quorum sensing (QS)-based dynamic regulation is widely used to automatically activate gene expression in response to changes in cell density. The main bottleneck currently limiting the use of exogenous QS systems in B. subtilis for efficient autoinducible extracellular expression of recombinant proteins is their low level of autoinducible expression.
resultsA novel B. subtilis autoinducible extracellular expression system based on the LuxI/R-type QS system (lux system) of Vibrio fischeri was developed in which the autoinducible expression of the lux system was enhanced by engineering the sensing module and response module promoters. By engineering the sensing module promoter SP
conclusionsTo our knowledge, this is the first study to report enhanced autoinducible expression of the lux system in B. subtilis by engineering the sensing module promoter SP
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