Evidence map›Paper›PMID 40251060›Full record

ReviewTrends in parasitology2025

Work with me here: variations in genome content and emerging genetic tools in Entamoeba histolytica.

Wesley Huang, Maura C Ruyechan, Katherine S Ralston

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Trends in parasitology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Wesley HuangDepartment of Microbiology and Molecular Genetics, University of California, Davis, CA, USA.
Maura C RuyechanDepartment of Microbiology and Molecular Genetics, University of California, Davis, CA, USA.
Katherine S RalstonDepartment of Microbiology and Molecular Genetics, University of California, Davis, CA, USA. Electronic address: ksralston@ucdavis.edu.

Funding

Comparative Medical Science Training ProgramT32OD011147 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI JEROEN SAEIJ, Sara Michelle Thomasy · 2012 to 2026
$5.5M
The role of Entamoeba histolytica trogocytosis (trogo-: nibble) in the pathogenesis of amoebiasisR01AI146914 · NIAID · UNIVERSITY OF CALIFORNIA AT DAVIS · PI RALSTON, KATHERINE S · 2019 to 2023
$1.9M
NIAID NIH HHS R01 AI146914NIH HHS T32 OD011147
6 · The paper itself

Abstract

Entamoeba histolytica is the causative agent of amoebiasis, a significant source of morbidity and mortality in developing nations. Despite this, E. histolytica is understudied, leading to few treatment options and a poor understanding of pathogenesis. Genetic tools have historically been limited. By applying modern approaches, it was recently revealed that the genome is aneuploid. Interestingly, gene expression levels do not correlate with ploidy, potentially highlighting the importance of RNAi in gene regulation. Characterization of the RNAi pathway has led to potent tools for targeted gene knockdown, and the advent of RNAi-based forward genetics. CRISPR/Cas tools for editing the endogenous genome are an exciting possibility on the horizon. We celebrate the gains that have made E. histolytica tractable and anticipate continued advances.

Indexed as

Entamoeba histolyticaGenetic VariationGenome, ProtozoanCRISPR-Cas SystemsEntamoebiasisHumansRNA InterferenceCRISPR/Cas9EntamoebapolyploidRNAi

Identifiers

PMID40251060
PMCPMC12640643

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.