Evidence map›Paper›PMID 40249438›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Thawing, Growth, and Maintenance of Vero Cell Lines.

Kelly Araujo Lúcio, Ivanielle Curvello Mendonça, Rafael Araújo Mendonça, Vanessa Oliveira Dos Santos

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Kelly Araujo LúcioLaboratory of Virological Technology (LATEV), Institute of Technology in Immunobiologicals (Bio-Manguinhos), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, RJ, Brazil. kelly.araujo@bio.fiocruz.br.
Ivanielle Curvello MendonçaLaboratory of Virological Technology (LATEV), Institute of Technology in Immunobiologicals (Bio-Manguinhos), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, RJ, Brazil.
Rafael Araújo MendonçaLaboratory of Virological Technology (LATEV), Institute of Technology in Immunobiologicals (Bio-Manguinhos), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, RJ, Brazil.
Vanessa Oliveira Dos SantosLaboratory of Virological Technology (LATEV), Institute of Technology in Immunobiologicals (Bio-Manguinhos), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, RJ, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vero cell line is derived from the kidney of an African green monkey and is one of the most common mammalian continuous cell lines used in research. Its deficiency in the production of type 1 interferons allows this cell line to be susceptible and permissible to replication of several viruses, including the yellow fever virus. Continuous cell culture in the laboratory can increase the risk of contamination, as well as loss of characteristics of interest in the strain. To minimize these risks a cultivation standard should be established. This chapter presents useful protocols for the maintenance of Vero cells for laboratorial routine, such as thawing vials from a cell bank stock, maintenance by subcultivation procedures, adaptation of the cells to grow in serum-free media, and production of cell culture plates for laboratory routine assays.

Indexed as

Cell Culture TechniquesCryopreservationAnimalsChlorocebus aethiopsCulture Media, Serum-FreeVero CellsCulture Media, Serum-FreeCell culture techniquesCell platingCell thawingContinuous cell linePDLSerum-free mediumVero cells

Identifiers

PMID40249438

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.