Evidence map›Paper›PMID 40249436›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Micro-Focus Reduction Neutralization Tests (mFRNT) for Neutralizing Antibody (Nab) Quantification Against Yellow Fever Virus.

Adriana de Souza Azevedo, Nathalia Dos Santos Alves

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Adriana de Souza AzevedoLaboratory of Immunomolecular Analysis, Institute of Technology in Immunobiologicals (Bio-Manguinhos), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil. adriana.soares@bio.fiocruz.br.
Nathalia Dos Santos AlvesLaboratory of Immunomolecular Analysis, Institute of Technology in Immunobiologicals (Bio-Manguinhos), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil. nathalia.alves@bio.fiocruz.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Although the plaque reduction neutralization test (PRNT) is considered the reference for measuring neutralizing antibodies for Yellow fever virus (YFV), the micro-focus reduction neutralization test (mFRNT) presents many advantages including a higher throughput, a reduced incubation time, and allows the automatic foci count. So, here we describe a detailed mFRNT method that provides a faster neutralization test essential for non-clinical and clinical studies and vaccine design.

Indexed as

Antibodies, NeutralizingAntibodies, ViralNeutralization TestsYellow FeverYellow fever virusAnimalsChlorocebus aethiopsHumansVero CellsViral Plaque AssayYellow Fever VaccineAntibodies, NeutralizingAntibodies, ViralYellow Fever VaccineFlavivirusFocusImmunoassaymFRNTNabNeutralizing antibodiesYellow fever virus

Identifiers

PMID40249436

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.