Evidence map›Paper›PMID 40249429›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Conventional RT-PCR for Yellow Fever Virus.

Nieli Rodrigues da Costa Faria, Renata Monteiro-Maia, Priscila Conrado Guerra Nunes, Monique da Rocha Queiroz Lima, Fernanda de Bruycker-Nogueira

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Nieli Rodrigues da Costa FariaLaboratory of Arboviruses and Hemorrhagic Viruses (LARBOH), Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, RJ, Brazil.
Renata Monteiro-MaiaLaboratory of Molecular Biology of Insects, Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, RJ, Brazil.
Priscila Conrado Guerra NunesLaboratory of Parasite-Host Interactions (LIVH), Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, RJ, Brazil.
Monique da Rocha Queiroz LimaStrategic Laboratory for Molecular Diagnostics, Butantan Institute, São Paulo, Brazil.
Fernanda de Bruycker-NogueiraLaboratory of Arboviruses and Hemorrhagic Viruses (LARBOH), Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, RJ, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The yellow fever virus (YFV), prototype of the genus Orthoflavivirus (family Flaviviridae), contains a positive-sense RNA genome that is translated as a single polyprotein which comprises approximately 11,000 nucleotides, encoding 3411 amino acids. In recent years, a number of molecular assays have been reported for YFV RNA detection, with different levels of sensitivity and specificity. Reverse transcription Polymerase Chain Reaction (RT-PCR) assays are the most frequent methods for YFV molecular diagnostics currently. Molecular methods of diagnostics for viral genome detection offer a fast, sensitive, and highly specific alternative to serological assays for early diagnostics during the viremic phase of infection or in fatal cases tissues. This chapter presents some conventional RT-PCR protocols used in molecular diagnostics, among the several available in the literature.

Indexed as

Reverse Transcriptase Polymerase Chain ReactionYellow FeverYellow fever virusGenome, ViralHumansRNA, ViralRNA, ViralDiagnosisMolecularNested RT-PCRRT-PCRYellow fever

Identifiers

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.