Evidence map›Paper›PMID 40249425›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Immunofluorescence Assay.

Andréa Nazaré Monteiro Rangel da Silva, José Valter Joaquim Silva Júnior

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Andréa Nazaré Monteiro Rangel da SilvaInstitute of Biological Sciences, Virology Laboratory, Federal University of Pará, Belém, Pará, Brazil.
José Valter Joaquim Silva JúniorDepartment of Microbiology and Parasitology, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The investigation of yellow fever suspected cases is important for both epidemiological surveillance and for the timely adoption of supportive therapy. The immunofluorescence assay (IFA), a technique based on the detection of specific targets by fluorescent dye-tagged antibodies, may be useful for the serological diagnosis of yellow fever and/or for monitoring of viral isolation. In addition, IFA can also be used for detection of yellow fever virus (YFV) antigens, as well as for recombinant YFV proteins after cell transfection. Here we describe a simple in-house IFA protocol for YFV, which may be useful for research purposes or laboratory diagnosis.

Indexed as

Fluorescent Antibody TechniqueYellow FeverYellow fever virusAnimalsAntibodies, ViralAntigens, ViralHumansAntibodies, ViralAntigens, ViralCell transfection monitoringDiagnosisFluorescent dye-tagged antibodyProtein detectionSerologyViral isolationYellow fever

Identifiers

PMID40249425

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.