Evidence map›Paper›PMID 40247828›Full record

ArticlePeerJ2025

Advancing molecular macrobenthos biodiversity monitoring: a comparison between Oxford Nanopore and Illumina based metabarcoding and metagenomics.

Karlijn Doorenspleet, Amalia Aikaterini Mailli, Berry B van der Hoorn, Kevin K Beentjes, Annelies De Backer, Sofie Derycke, Albertinka J Murk, Henning Reiss, Reindert Nijland

Abstract readComparative Study
In one paragraph

Article in PeerJ, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Metagenomic next-generation sequencing: new horizons in microbiology.Frontiers in cellular and infection microbiology · 2026
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Karlijn Doorenspleet *Marine Animal Ecology, Wageningen University and Research, Wageningen, Netherlands.
Amalia Aikaterini Mailli *Faculty of Biosciences and Aquaculture, Nord University, Bodø, Norway.
Berry B van der HoornNaturalis Biodiversity Center, Leiden, Netherlands.
Kevin K BeentjesNaturalis Biodiversity Center, Leiden, Netherlands.
Annelies De BackerILVO Marine Research, Flanders Research Institute for Agriculture, Fisheries and Food, Oostende, Belgium.
Sofie DeryckeILVO Marine Research, Flanders Research Institute for Agriculture, Fisheries and Food, Oostende, Belgium.
Albertinka J MurkMarine Animal Ecology, Wageningen University and Research, Wageningen, Netherlands.
Henning ReissFaculty of Biosciences and Aquaculture, Nord University, Bodø, Norway.
Reindert NijlandMarine Animal Ecology, Wageningen University and Research, Wageningen, Netherlands.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA-based methods and developments of sequencing technologies are integral to macrobenthos biodiversity studies, and their implementation as standardized monitoring methods is approaching. Evaluating the efficacy and reliability of these technological developments is crucial for macrobenthos biodiversity assessments. In this study, we compared three DNA-based techniques for assessing the diversity of bulk macrobenthos samples from the Belgian North Sea. Specifically, we compared amplicon sequencing using Illumina MiSeq and portable real-time sequencing of Oxford Nanopore

Indexed as

BiodiversityDNA Barcoding, TaxonomicMetagenomicsBelgiumHigh-Throughput Nucleotide SequencingNanoporesNorth SeaBenthic communitiesDNAIlluminaMetabarcodingMetagenomicsMiseqNanoporeNorth SeaNovaSeq

Identifiers

PMID40247828
PMCPMC12005195

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.