ArticleInsect science2026
The role of BmsPLA2-4 in tissue defense against pathogens in silkworm, Bombyx mori.
Article in Insect science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- The Relative Contributions ofInsects · 2026Article
- CRISPR-Mediated Silkworm: The Oncoming Agricultural Revolutions and a Rising Model Organism.Genes · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Lepidoptera, one of the largest orders within the class Insecta, accounts for approximately 70% of known pests affecting crops and forests. Therefore, immune research in the lepidopteran model Bombyx mori offers valuable insights for refining pest control strategies. Although numerous studies have investigated the role of phospholipase A2 (PLA2) in immunity, research on its role in tissues remains lacking in insects. We previously found that BmsPLA2-4 is highly expressed in the midgut, a key immune tissue in silkworms. In this study, we successfully generated BmsPLA2-4 overexpressing and knockout transgenic strains using the piggyBac system, microinjection, and fluorescence screening. The target sequence was inserted into NSCAF6497 on chromosome 15 in overexpressing individuals, and deletions or mutations of 13 or 14 bp occurred near genomic guide RNA targets in knockout individuals. Upon oral bacterial challenge, midgut bacterial load was significantly reduced in overexpressing individuals and markedly elevated in knockout individuals. Furthermore, bacterial growth was significantly promoted by hemolymph from knockout individuals. Expression levels of various immunity-related genes in the midgut were significantly altered in transgenic individuals following pathogen infection. These suggested that BmsPLA2-4 plays an important role in immune tissue resistance to pathogens in silkworm. Interestingly, BmsPLA2-4 protein expressed in vitro in eukaryotic systems inhibits bacterial growth without binding to bacteria, providing new data for studying the mechanism of BmsPLA2-4 in immunity. Our work, for the first time in insects, demonstrates PLA2's role in tissues against pathogens, offering reliable data for applications in silkworms or other insects.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.