ArticleCell communication and signaling : CCS2025
Nicotinamide mononucleotide biosynthesis and the F-actin cytoskeleton regulate spindle assembly and oocyte maturation quality in post-ovulatory aged porcine oocytes.
Article in Cell communication and signaling : CCS, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed.
- MK-4 Ameliorates Post-Ovulatory Aging of Mouse Oocytes by Alleviating Iron Metabolism Disorder-Induced Oxidative Stress.Molecular reproduction and development · 2026Article
- EGR1 Mediates Ursodeoxycholic Acid-Promoted Mitophagy to Prevent Postovulatory Aging of Porcine Oocytes.Aging cell · 2026Article
- Liquiritigenin Restores Declined Oocyte Quality and Improves Embryo Development in Aged Mice.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
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6 authors.
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Abstract
backgroundPost-ovulatory aging (POA) is associated with reduced fertilization rates and poor embryo quality both in vivo and in vitro. However, the relationship between nicotinamide adenine dinucleotide (NAD
methodsPorcine oocytes were aged by extending in vitro maturation (IVM) for an additional 24 h to create a POA model. F-actin and adducin 1 (ADD1)-related spindle assembly were analyzed using immunofluorescence, western blotting, and RNA sequencing to identify key gene categories in the POA and IVM groups. To assess NAD
resultsWe revealed unique interactions between the F-actin/ADD1-related cytoskeleton and aging factors (clusterin (CLU) and FAM111 trypsin-like peptidase A (FAM111A)) in poor-quality oocytes. POA oocytes were established with an extension of 24 h based on 44 h of IVM. They exhibited actin collapses and abnormal cortical F-actin, ADD1, and acetyl(Ac)-α-tubulin protein levels, which resulted in defective spindle assembly. RNA sequencing analysis was performed to identify differentially expressed genes involved in the oocyte viability response to aging, the cytoskeleton, and NAD metabolic processes using IVM and/or POA oocytes. This showed that NAD-binding genes were differentially expressed after POA induction, eight of which were downregulated compared with IVM oocytes. Importantly, activation of NAD
conclusionF-actin polymerization through NAD
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