Evidence map›Paper›PMID 40247144›Full record

ArticleNature plants2025

Enrichment of two important metabolites D-galacturonic acid and D-glucuronic acid inhibits MdHb1-mediated fruit softening in apple.

Yu-Wen Zhao, Ting-Ting Zhao, Quan Sun, Xiao-Long Liu, Xiao-Yu Huang, Lin-Guang Li, Hai-Bo Wang, Wan-Kun Li, Chu-Kun Wang, Wen-Yan Wang and 5 more

Abstract read
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In one paragraph

Article in Nature plants, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
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  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Yu-Wen Zhao *National Research Center for Apple Engineering and Technology; Shandong Collaborative Innovation Center of Fruit and Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, China.
Ting-Ting Zhao *National Research Center for Apple Engineering and Technology; Shandong Collaborative Innovation Center of Fruit and Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, China.
Quan Sun *National Research Center for Apple Engineering and Technology; Shandong Collaborative Innovation Center of Fruit and Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, China.
Xiao-Long Liu *National Research Center for Apple Engineering and Technology; Shandong Collaborative Innovation Center of Fruit and Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, China.
Xiao-Yu HuangNational Research Center for Apple Engineering and Technology; Shandong Collaborative Innovation Center of Fruit and Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, China.
Lin-Guang LiShandong Institute of Pomology, Key Laboratory for Fruit Biotechnology Breeding of Shandong, Tai'an, Shandong, China.
Hai-Bo WangShandong Institute of Pomology, Key Laboratory for Fruit Biotechnology Breeding of Shandong, Tai'an, Shandong, China.
Wan-Kun LiNational Research Center for Apple Engineering and Technology; Shandong Collaborative Innovation Center of Fruit and Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, China.
Chu-Kun WangNational Research Center for Apple Engineering and Technology; Shandong Collaborative Innovation Center of Fruit and Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, China.ORCID 0000-0001-6640-5180
Wen-Yan WangNational Research Center for Apple Engineering and Technology; Shandong Collaborative Innovation Center of Fruit and Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, China.
Ying XiangDepartment of Horticulture, Agriculture College, Shihezi University, Shihezi, China.
Chang-Ning MaNational Research Center for Apple Engineering and Technology; Shandong Collaborative Innovation Center of Fruit and Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, China.
Xue-Sen ChenNational Research Center for Apple Engineering and Technology; Shandong Collaborative Innovation Center of Fruit and Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, China.
Lailiang ChengSection of Horticulture, School of Integrative Plant Science, Cornell University, Ithaca, NY, USA.
Da-Gang HuNational Research Center for Apple Engineering and Technology; Shandong Collaborative Innovation Center of Fruit and Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, China. fap_296566@163.com.ORCID 0000-0002-1443-3646

Funding

National Natural Science Foundation of China (National Science Foundation of China) 32122080Taishan Scholar Project of Shandong Province tsqnz20231206
6 · The paper itself

Abstract

In apples, fruit firmness is a crucial quality trait influencing fruit storability, transportability, shelf life and consumer preference. However, the genetic network underlying this trait remains unclear. Therefore, the present study investigated the changes in apple fruit at different stages of postharvest storage using a combination of transcriptomic and metabolomic analyses. With prolonged storage, we detected a significant increase in two metabolites, D-galacturonic acid (D-GalUA) and D-glucuronic acid (D-GlcA), which are associated with a key class 1 non-symbiotic haemoglobin (MdHb1). We innovatively found that MdHb1 regulates fruit softening by catalysing the conversion from protopectin to water-soluble pectin. Biochemical analysis demonstrated that MdMYB2/MdNAC14/MdNTL9 transcription factors directly bind to the MdHb1 promoter to activate its transcriptional expression and promote fruit softening. Further injection experiments in apple fruit and histological as well as transmission electron microscopy analyses of the fruit samples revealed that D-GalUA and D-GlcA reduce the transcription of MdHb1, or through the MdMYB2/MdNAC14/MdNTL9-MdHb1 regulatory module, thereby delaying fruit softening. Our study provides novel insights into the role of two important metabolites, D-GalUA and D-GlcA, in the regulation of MdHb1-mediated fruit softening in apples.

Indexed as

FruitHexuronic AcidsMalusPlant ProteinsGene Expression Regulation, PlantPectinsTranscription Factorsgalacturonic acidHexuronic AcidsPectinsPlant ProteinsTranscription Factors

Identifiers

PMID40247144

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.