ArticleNature methods2025
A highly stable monomeric red fluorescent protein for advanced microscopy.
Article in Nature methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
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- Organelle-specific fluorescent probes for live imaging at the nanoscale.Science China. Life sciences · 2026Review
- A cytosolic IF1 reporter enables real-time visualization of severe mitochondrial membrane damage.The Journal of cell biology · 2026Article
- 3D Single-Virus Tracking: Advances in Methodology and Labeling Strategies Towards Probing the Virus-Epithelium Interaction.Viruses · 2026Review
- Dark-State-Mediated Photobleaching in mCherry-Based Red Fluorescent Proteins.The journal of physical chemistry letters · 2026Article
- A ratiometric fluorescent dual-mode amplification nanosensor based on pH and oxygen testing for ultrasensitive glucose detection.Mikrochimica acta · 2026Article
- A fluorescent reporter system for trackingFrontiers in microbiology · 2026Article
- Synaptic output from suprachiasmatic nucleus cholecystokinin neurons regulates locomotor rhythmicity.Frontiers in neuroscience · 2026Article
- BRIGHT Enables High-SNR Live-Cell Imaging of Non-Repetitive Sequences via Bivalent Fluorescent Nanobody-Mediated Cascade-Dependent Illumination.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A highly photostable monomeric red fluorescent protein for dual-color 3D STED and time-lapse 3D SIM imaging.Nature methods · 2026Article
- Year in review 2025.Nature methods · 2026Article
- MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox for use in vertebrate model systems.Development (Cambridge, England) · 2025Article
- Lysosomal and mTORC1 signaling dysregulation underpin the pathology of spastic paraplegia type 80.Nature communications · 2025Article
- Fluorescent protein tags for human tropomyosin isoform comparison.Biology open · 2025Article
- Quantitative comparison of fluorescent proteins using protein nanocages in live cells.Journal of cell science · 2025Article
- Capturing more than meets the eye.Nature methods · 2025Article
Corrections and comments
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Authors and funding
32 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The stability of fluorescent proteins (FPs) is crucial for imaging techniques such as live-cell imaging, super-resolution microscopy and correlative light and electron microscopy. Although stable green and yellow FPs are available, stable monomeric red FPs (RFPs) remain limited. Here we develop an extremely stable monomeric RFP named mScarlet3-H and determine its structure at a 1.5 Å resolution. mScarlet3-H exhibits remarkable resistance to high temperature, chaotropic conditions and oxidative environments, enabling efficient correlative light and electron microscopy imaging and rapid (less than 1 day) whole-organ tissue clearing. In addition, its high photostability allows long-term three-dimensional structured illumination microscopy imaging of mitochondrial dynamics with minimal photobleaching. It also facilitates dual-color live-cell stimulated emission depletion imaging with a high signal-to-noise ratio and strong specificity. Systematic benchmarking against high-performing RFPs established mScarlet3-H as a highly stable RFP for multimodality microscopy in cell cultures and model organisms, complementing green FPs for multiplexed imaging in zebrafish, mice and Nicotiana benthamiana.
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