ReviewNature methods2025
Imaging 3D cell cultures with optical microscopy.
Review in Nature methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed.
- Illuminating the Interface: In Situ Optical Insights Into Two-Electron ORR Pathways for HAdvanced materials (Deerfield Beach, Fla.) · 2026Review
- Extracellular matrix-mimic nanofibrous microspheres: fabrication and application in stem cell delivery for tissue repair and regeneration.Materials today. Bio · 2026Review
- Multiparametric dynamic optical coherence tomography for evaluation of human cardiac organoids subjected to external stress.Biomedical optics express · 2026Article
- A self-organized pencil beam that can be used for fast volumetric imaging.Nature methods · 2026Article
- Morphology-coupled formation and reversible gating of membrane channels in synthetic cells using reconfigurable DNA nanorafts.Nature protocols · 2026Review
- Strategies to control cellular spatial organization in microphysiological systems.Microsystems & nanoengineering · 2026Review
- An Intelligent Magneto-Mechanical Platform for Cellular Sensing in 3D Microenvironments.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Engineering Aging: Approaches to Model and Deconstruct Biological Complexity.Advanced materials (Deerfield Beach, Fla.) · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
Abstract
Three-dimensional (3D) cell cultures have gained popularity in recent years due to their ability to represent complex tissues or organs more faithfully than conventional two-dimensional (2D) cell culture. This article reviews the application of both 2D and 3D microscopy approaches for monitoring and studying 3D cell cultures. We first summarize the most popular optical microscopy methods that have been used with 3D cell cultures. We then discuss the general advantages and disadvantages of various microscopy techniques for several broad categories of investigation involving 3D cell cultures. Finally, we provide perspectives on key areas of technical need in which there are clear opportunities for innovation. Our goal is to guide microscope engineers and biomedical end users toward optimal imaging methods for specific investigational scenarios and to identify use cases in which additional innovations in high-resolution imaging could be helpful.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.