Evidence map›Paper›PMID 40247007›Full record

ArticleCommunications biology2025

Near-infrared spectroscopy as a diagnostic screening tool for lethal chytrid fungus in eastern newts.

Li-Dunn Chen, Edward D Carter, Merrie P Urban, Carmen T Merolle, Devin M Chen, Andrew J Kouba, Matthew J Gray, Debra L Miller, Carrie K Kouba

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Li-Dunn ChenDepartment of Biochemistry, Molecular Biology, Entomology & Plant Pathology, Mississippi State University, Mississippi State, MS, USA.ORCID http://orcid.org/0000-0002-9653-9814
Edward D CarterCenter for Wildlife Health, School of Natural Resources, University of Tennessee, Knoxville, TN, USA.ORCID http://orcid.org/0000-0001-6187-9010
Merrie P UrbanCenter for Wildlife Health, School of Natural Resources, University of Tennessee, Knoxville, TN, USA.
Carmen T MerolleCenter for Wildlife Health, School of Natural Resources, University of Tennessee, Knoxville, TN, USA.
Devin M ChenDepartment of Wildlife, Fisheries, & Aquaculture, Mississippi State University, Mississippi State, MS, USA.ORCID http://orcid.org/0000-0001-6418-3801
Andrew J KoubaDepartment of Wildlife, Fisheries, & Aquaculture, Mississippi State University, Mississippi State, MS, USA.
Matthew J GrayCenter for Wildlife Health, School of Natural Resources, University of Tennessee, Knoxville, TN, USA.ORCID http://orcid.org/0000-0001-8243-9217
Debra L MillerCenter for Wildlife Health, School of Natural Resources, University of Tennessee, Knoxville, TN, USA.ORCID http://orcid.org/0000-0002-8544-174X
Carrie K KoubaDepartment of Biochemistry, Molecular Biology, Entomology & Plant Pathology, Mississippi State University, Mississippi State, MS, USA. ckv7@msstate.edu.ORCID http://orcid.org/0000-0001-8466-6077

Funding

Institute of Museum and Library Services (IMLS) #MG-251614-OMS-22Institute of Museum and Library Services (IMLS) #MG-30-17-0052-17National Science Foundation (NSF) #DEB EEID grant 1814520National Science Foundation (NSF) DEB EEID grant 1814520United States Department of Agriculture | Agricultural Research Service (USDA Agricultural Research Service) #6066-31000-015-00DUnited States Department of Agriculture | U.S. Forest Service (Forest Service) #18-DG-11132762-248U.S. Department of Agriculture (United States Department of Agriculture) NIFA Hatch project #1012932
6 · The paper itself

Abstract

The emergence of Batrachochytrium salamandrivorans (Bsal) poses an imminent threat to caudate biodiversity worldwide, particularly through anthropogenic-mediated means such as the pet trade. Bsal is a fungal panzootic that has yet to reach the Americas, Africa, and Australia, presenting a significant biosecurity risk to naïve amphibian populations lacking the innate immune defenses necessary for combating invasive pathogens. We explored the capability of near-infrared spectroscopy (NIRS) coupled with predictive modeling as a rapid, non-invasive Bsal screening tool in live caudates. Using eastern newts (Notopthalmus viridescens) as a model species, NIR spectra were collected in tandem with dermal swabs used for confirmatory qPCR analysis. We identified that spectral profiles differed significantly by physical location (chin, cloaca, tail, and foot) as well as by Bsal pathogen status (control vs. exposed individuals; p < 0.05). The support vector machine algorithm achieved a mean classification accuracy of 80% and a sensitivity of 92% for discriminating Bsal-control (-) from Bsal-exposed (+) individuals. This approach offers a promising method for identifying Bsal-compromised populations, potentially aiding in early detection and mitigation efforts alongside existing techniques.

Indexed as

BatrachochytriumMycosesAnimalsSpectroscopy, Near-InfraredSupport Vector Machine

Identifiers

PMID40247007
PMCPMC12006519

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.