Evidence map›Paper›PMID 40246794›Full record

ArticleAngewandte Chemie (International ed. in English)2025

Functional Dendrimer Nanogels for DNA Delivery and Gene Therapy of Tumors.

Xin Li, Zhijun Ouyang, Laura Hetjens, Ming Ni, Kuailu Lin, Yong Hu, Xiangyang Shi, Andrij Pich

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xin Li *Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, 52074, Aachen, Germany.
Zhijun Ouyang *College of Biological Science and Medical Engineering, Donghua University, Shanghai, 201620, China.
Laura HetjensInstitute of Technical and Macromolecular Chemistry, RWTH Aachen University, 52074, Aachen, Germany.
Ming NiDepartment of Orthopaedics, Rujin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
Kuailu LinDepartment of Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015, China.
Yong HuDepartment of Polymeric Materials, School of Materials Science and Engineering, Tongji University, Shanghai, 201804, China.
Xiangyang ShiCollege of Biological Science and Medical Engineering, Donghua University, Shanghai, 201620, China.
Andrij PichInstitute of Technical and Macromolecular Chemistry, RWTH Aachen University, 52074, Aachen, Germany.

Funding

China Scholarship CouncilDeutsche Forschungsgemeinschaft SPP 2416National Key R&D Program 2022YFE010205National Natural Science Foundation of China 52103181National Science Foundation of China Research Fund for International Scientists 52350710203Science and Technology Project of Wenzhou Y2023489Sino-German Center for Research Promotion GZ1505
6 · The paper itself

Abstract

Solving the dilemma between efficacy and cytotoxicity of cationic colloidal vectors is one of the biggest challenges in gene delivery. Cationic dendrimer assemblies with hierarchical structure, smart and biomimetic behaviors have been developed for drug/gene delivery in vivo. Among different dendrimer assemblies, the dendrimer-based nanogels were not intensively studied due to complicated synthesis and unknown properties. Here, for the first time, low-generation dendrimer nanogels with high yield and purity, tunable size, uniform morphology, and good colloidal stability were synthesized using the emulsion-free method, which cannot be obtained by the miniemulsion method. Importantly, the dendrimer nanogels integrate the advantages of low-generation dendrimer and stimuli-responsive polymer, thus achieving dual-active groups, o-hydroxyl amine units, temperature-responsiveness, polyampholyte property, and self-triggered aminolysis. With these unique properties, dendrimer nanogels can "temporarily" acquire high charge density through the covalent crosslinking of low-generation dendrimer for improved DNA compression, promoted cell internalization and lysosomal escape, and efficient DNA delivery, followed by self-triggered aminolysis into small dendrimers to control DNA release, reduce cytotoxicity, and facilitate metabolism in vivo. Compared to high-generation dendrimers, low-generation dendrimer nanogels display higher gene transfection and therapeutic efficacies, and lower side effects simultaneously. This work provides a facile strategy for the preparation of low-generation dendrimer nanogels that break up the contradiction between efficacy and cytotoxicity of cationic colloidal vectors in gene therapy. This innovative approach to construct low-generation dendrimers into smart dendrimer nanogels will have broad applicability in clinical translation.

Indexed as

DendrimersDNAGenetic TherapyGene Transfer TechniquesNeoplasmsPolyethylene GlycolsPolyethyleneimineAnimalsHumansMiceNanogelsDendrimersDNANanogelsPolyethylene GlycolsPolyethyleneimineDendrimer nanogelsDNA deliveryEmulsion‐freeMultiresponsivenessSelf‐triggered degradation

Identifiers

PMID40246794
PMCPMC12281078

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.