Evidence map›Paper›PMID 40244850›Full record

ArticleCell reports2025

PP1/PNUTS phosphatase binds the restrictor complex and stimulates RNA Pol II transcription termination.

Benjamin Erickson, Roman Fedoryshchak, Nova Fong, Ryan Sheridan, Keira Y Larson, Anthony J Saviola, Stephane Mouilleron, Kirk C Hansen, Richard Treisman, David L Bentley

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
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  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Benjamin EricksonDepartment of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA.
Roman FedoryshchakFrancis Crick Institute, Midland Road, London UK.
Nova FongDepartment of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA.
Ryan SheridanRNA Bioscience Initiative, Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA.
Keira Y LarsonDepartment of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA.
Anthony J SaviolaDepartment of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA.
Stephane MouilleronFrancis Crick Institute, Midland Road, London UK.
Kirk C HansenDepartment of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA.
Richard TreismanFrancis Crick Institute, Midland Road, London UK. Electronic address: richard.treisman@crick.ac.uk.
David L BentleyRNA Bioscience Initiative, Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA. Electronic address: david.bentley@cuanschutz.edu.

Funding

Coupling of transcription elongation and termination with pre-mRNA processingR35GM144336 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI DAVID L BENTLEY · 2022 to 2026
$2.5M
Coupling of transcription with nascent pre-mRNA metabolismR35GM118051 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI BENTLEY, DAVID L · 2016 to 2020
$2.2M
Cancer Research UK CC2102NIGMS NIH HHS R35 GM118051NIGMS NIH HHS R35 GM144336Wellcome Trust CC2102
6 · The paper itself

Abstract

The restrictor ZC3H4/WDR82 terminates antisense transcription from bidirectional promoters, but its mechanism is poorly understood. We report that ZC3H4/WDR82 immunoprecipitates with PP1 phosphatase and its nuclear targeting subunit, PP1 phosphatase nuclear targeting subunit (PNUTS), which binds to WDR82. AlphaFold predicts a complex of PP1/PNUTS with the restrictor where both PNUTS and ZC3H4 contact WDR82. A substrate trap, PP1

Indexed as

DNA-Binding ProteinsNuclear ProteinsProtein Phosphatase 1RNA-Binding ProteinsRNA Polymerase IITranscription Termination, GeneticHEK293 CellsHeLa CellsHumansPhosphorylationProtein BindingTranscription, GeneticDNA-Binding ProteinsNuclear ProteinsProtein Phosphatase 1RNA-Binding ProteinsRNA Polymerase IICP: Molecular biologyCTD dephosphorylationPol II CTD Ser5 phosphorylationPP1 phosphataserestrictortranscription elongationtranscription terminationWDR82ZC3H4

Identifiers

PMID40244850
PMCPMC12187157

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.