Evidence map›Paper›PMID 40244445›Full record

ArticleMycopathologia2025

Deciphering Fluconazole Resistance in Candida auris clade V: The Role of Efflux Pump Gene Expression and Ergosterol Pathway Mutations.

Robab Ebrahimi Barough, Mahdi Abastabar, Maryam Moazeni, Javad Javidnia, Reza Valadan, Azadeh Bandegani, Mohsen Nosratabadi, Iman Haghani, Bram Spruijtenburg, Darius Armstrong-James and 1 more

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Article in Mycopathologia, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Robab Ebrahimi BaroughStudent Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.
Mahdi AbastabarInvasive Fungi Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, 48157-33971, Iran. mabastabar@gmail.com.
Maryam MoazeniInvasive Fungi Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, 48157-33971, Iran.
Javad JavidniaInvasive Fungi Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, 48157-33971, Iran.
Reza ValadanDepartment of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Azadeh BandeganiInvasive Fungi Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, 48157-33971, Iran.
Mohsen NosratabadiDepartment of Laboratory Sciences, Sirjan School of Medical Sciences, Sirjan, Iran.
Iman HaghaniInvasive Fungi Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, 48157-33971, Iran.
Bram SpruijtenburgRadboudumc-CWZ Center of Expertise for Mycology, Nijmegen, The Netherlands.
Darius Armstrong-JamesDepartment of Infectious Diseases, Medical Research Council Centre for Molecular Bacteriology and Infection, Imperial College London, London, UK.
Hamid BadaliDepartment of Molecular Microbiology & Immunology/South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, TX, USA. hamid.badali@utsa.edu.ORCID http://orcid.org/0000-0002-6010-8414

Funding

Mazandaran University of Medical Sciences 9431
6 · The paper itself

Abstract

Candida auris is an emerging multidrug-resistant yeast pathogen that poses a serious global health threat. In particular, fluconazole resistance is common in C. auris, posing challenges for treating invasive infections. Understanding the genetic and molecular mechanisms underlying fluconazole resistance in C. auris is crucial for developing effective control strategies. The current study investigated the genetic and molecular basis of fluconazole resistance in C. auris clade V isolates. Furthermore, we examined mutations in ergosterol biosynthesis genes and expression of efflux pump genes in fluconazole-resistant versus susceptible in strains Clade V. Two C. auris isolates, one fluconazole-resistant, and one fluconazole-susceptible, were subjected to qPCR analysis of efflux pump gene (CDR1, CDR2, MDR1, MDR2) expression. Protein structure modeling was also performed to assess the impact of mutation in the ergosterol biosynthesis gene (ERG11) on antifungal drug accessibility. qPCR analysis revealed no significant difference in the expression levels of the efflux pump genes CDR1, CDR2, and MDR1 between the resistant and susceptible strains. Protein structure modeling indicated that the Y132F mutation in ERG11 likely altered fluconazole binding and accessibility. This study provides insights into the genetic and molecular mechanisms underpinning fluconazole resistance in C. auris Clade V. The findings highlight the critical roles of ERG11 mutation in mediating azole resistance in this emerging fungal pathogen.

Indexed as

Antifungal AgentsCandida aurisDrug Resistance, FungalErgosterolFluconazoleMembrane Transport ProteinsFungal ProteinsGene Expression ProfilingHumansMicrobial Sensitivity TestsModels, MolecularMutationReal-Time Polymerase Chain ReactionAntifungal AgentsErgosterolFluconazoleFungal ProteinsMembrane Transport ProteinsC. auris Clade VEfflux pump genesERG11Ergosterol biosynthesis genesGene expression analysis

Identifiers

PMID40244445

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.