Evidence map›Paper›PMID 40244133›Full record

ArticleInternational journal of molecular sciences2025

Fluorescence Resonance Energy Transfer for Drug Loading Assessment in Reconstituted High-Density Lipoprotein Nanoparticles.

R Max Petty, Luca Ceresa, Emma Alexander, Danh Pham, Nirupama Sabnis, Rafal Fudala, Andras G Lacko, Raghu R Krishnamoorthy, Zygmunt Gryczynski, Ignacy Gryczynski

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

R Max PettyDepartment of Pharmacology and Neuroscience, North Texas Eye Research Institute, Graduate School of Biomedical Science, University of North Texas Health Science Center, Fort Worth, TX 76107, USA.ORCID 0009-0007-3171-1066
Luca CeresaDepartment of Physics and Astronomy, Texas Christian University, Fort Worth, TX 76109, USA.ORCID 0000-0001-8771-496X
Emma AlexanderDepartment of Physics and Astronomy, Texas Christian University, Fort Worth, TX 76109, USA.ORCID 0000-0002-9226-2432
Danh PhamDepartment of Physics and Astronomy, Texas Christian University, Fort Worth, TX 76109, USA.ORCID 0000-0003-0274-9640
Nirupama SabnisDepartment of Physiology and Anatomy, Graduate School of Biomedical Sciences, University of North Texas Health Science Center, Fort Worth, TX 76107, USA.ORCID 0000-0002-8686-1578
Rafal FudalaDepartment of Physiology and Anatomy, Graduate School of Biomedical Sciences, University of North Texas Health Science Center, Fort Worth, TX 76107, USA.
Andras G LackoDepartment of Microbiology, Immunology, and Genetics, School of Biomedical Sciences, University of North Texas Health Science Center, Fort Worth, TX 76107, USA.
Raghu R KrishnamoorthyDepartment of Pharmacology and Neuroscience, North Texas Eye Research Institute, Graduate School of Biomedical Science, University of North Texas Health Science Center, Fort Worth, TX 76107, USA.
Zygmunt GryczynskiDepartment of Physics and Astronomy, Texas Christian University, Fort Worth, TX 76109, USA.
Ignacy GryczynskiDepartment of Physics and Astronomy, Texas Christian University, Fort Worth, TX 76109, USA.

Funding

Training in the Neurobiology of Aging and Alzheimer's DiseaseT32AG020494 · NIA · UNIVERSITY OF NORTH TEXAS HLTH SCI CTR · PI ROBERT Clinton BARBER, NATHALIE SUMIEN · 2002 to 2026
$6.9M
Development of Reconstituted High-Density Lipoprotein Nanoparticles for Targeted Delivery of Neuroprotective Agents to Retinal Ganglion CellsF31EY035916 · NEI · UNIVERSITY OF NORTH TEXAS HLTH SCI CTR · PI PETTY, ROLAND MAX · 2024 to 2025
$72k
NEI NIH HHS F31 EY035916NIA NIH HHS T32 AG020494NIH HHS 1F31EY035916-01X1NIH HHS 1T32AG020494-01X1
6 · The paper itself

Abstract

Reconstituted high-density lipoprotein nanoparticles (NPs), which mimic the structure and function of endogenous human plasma HDL, hold promise as a robust drug delivery system. These nanoparticles, when loaded with appropriate agents, serve as powerful tools for targeted drug delivery. The fundamental challenge lies in controlling and estimating the actual drug load and the efficiency of drug release at the target. In this report, we present a novel approach based on enhanced Förster Resonance Energy Transfer (FRET) to assess particle load and monitor payload release. The NPs are labeled with donor molecules embedded in the lipid phase, while the spherical core volume is filled with acceptor molecules. Highly enhanced FRET efficiency to multiple acceptors in the NP core has been observed at distances significantly larger than the characteristic Förster distance (R

Indexed as

Drug Delivery SystemsFluorescence Resonance Energy TransferLipoproteins, HDLNanoparticlesHumansLipoproteins, HDLdrug deliveryfluorescence lifetimeFRETlipoproteinsnanoparticles

Identifiers

PMID40244133
PMCPMC11989292

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.