Evidence map›Paper›PMID 40244033›Full record

ArticleInternational journal of molecular sciences2025

Morphological Characterization of Intrafollicular Epithelial Bodies (IFEBs) in Rabbit Peyer's Patches.

Tiziana Tamborrino, Denise Bonente, Marì Regoli, Valentina Costa, Virginia Barone, Emiliana Giacomello, Giulia Collodel, Niccolò Fagni, Claudio Nicoletti, Eugenio Bertelli

Abstract read
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In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Tiziana TamborrinoDepartment of Molecular and Developmental Medicine, University of Siena, Via A. Moro, 2, 53100 Siena, Italy.ORCID 0009-0000-7100-981X
Denise BonenteDepartment of Molecular and Developmental Medicine, University of Siena, Via A. Moro, 2, 53100 Siena, Italy.ORCID 0000-0002-3080-6406
Marì RegoliDepartment of Molecular and Developmental Medicine, University of Siena, Via A. Moro, 2, 53100 Siena, Italy.
Valentina CostaDepartment of Molecular and Developmental Medicine, University of Siena, Via A. Moro, 2, 53100 Siena, Italy.ORCID 0009-0000-8560-8805
Virginia BaroneDepartment of Molecular and Developmental Medicine, University of Siena, Via A. Moro, 2, 53100 Siena, Italy.ORCID 0000-0001-8794-9291
Emiliana GiacomelloDepartment of Medicine, Surgery and Health Sciences, University of Trieste, Strada Fiume, 447, 34149 Trieste, Italy.ORCID 0000-0003-3329-6269
Giulia CollodelDepartment of Molecular and Developmental Medicine, University of Siena, Via A. Moro, 2, 53100 Siena, Italy.ORCID 0000-0003-1587-0159
Niccolò FagniOtorinolaringoiatry, AOUS (Azienda Ospedaliero-Universitaria Senese), 53100 Siena, Italy.
Claudio NicolettiDepartment of Molecular and Developmental Medicine, University of Siena, Via A. Moro, 2, 53100 Siena, Italy.ORCID 0000-0002-6242-4867
Eugenio BertelliDepartment of Molecular and Developmental Medicine, University of Siena, Via A. Moro, 2, 53100 Siena, Italy.ORCID 0000-0002-9681-4556

Funding

Università di Siena PSR, grant n. 2267-2024-BV-PAR_001
6 · The paper itself

Abstract

Follicle-associated epithelium (FAE) covering the lymphoid follicles of Peyer's patches (PPs) plays a central role in mucosal immunity. Here, we investigated FAE-derived intrafollicular epithelial bodies (IFEBs) that apparently detach from the FAE and sink deep into the lymphoid tissue of the PPs. Analysis of rabbit PP FAE was carried out by a variety of microscopy and immunohistochemistry techniques using a panel of specific antibodies to determine the nature of the IFEBs. IFEBs displayed the typical features of the FAE, with cytokeratin (CK)+ epithelial cells and CK+/vimentin+ M-cell-like cells. Serial sections of PP tissues showed that the IFEBs are formations frequently separated by the overlying FAE that maintains its integrity. Further, IFEBs showed the presence of junction-associated molecules like zonulin-1 and desmoplakins. Also, IFEBs apparently disaggregate within the lymphoid tissue, as demonstrated by basement membrane disappearance and the finding of isolated epithelial cells that acquire the features of non-polarized epithelial cells. Segments of the FAE in rabbit PPs can detach, forming IFEBs that migrate inside the lymphoid tissue. Although the biological relevance of the newly described IFEBs remains to be determined, we interpreted these data as showing the highly dynamic nature of the PP-associated FAE.

Indexed as

Epithelial CellsPeyer's PatchesAnimalsEpitheliumImmunohistochemistryRabbitsanoikisbasement membranecell polarityentactinfollicle-associated epitheliumintrafollicular epithelial bodyM cells

Identifiers

PMID40244033
PMCPMC11989947

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.