Evidence map›Paper›PMID 40244028›Full record

ArticleInternational journal of molecular sciences2025

Microbiota of Healthy Dental Pulp Under the Omics Loupe.

Alan Bérard, Florian Mauffrey, Nadia Gaïa, Alexandre Perez, Chiraz Chaabane, Jacques Schrenzel, Julian Grégoire Leprince, Serge Bouillaguet, Vladimir Lazarevic

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Alan BérardDivision of Cariology and Endodontology, University Clinics of Dental Medicine (CUMD), University of Geneva, 1211 Geneva, Switzerland.
Florian MauffreyGenomic Research Laboratory, Division of Infectious Diseases, Department of Medicine, Geneva University Hospitals, University of Geneva, 1205 Geneva, Switzerland.
Nadia GaïaGenomic Research Laboratory, Division of Infectious Diseases, Department of Medicine, Geneva University Hospitals, University of Geneva, 1205 Geneva, Switzerland.
Alexandre PerezUnit of Oral Surgery and Implantology, Division of Oral and Maxillofacial Surgery, Department of Surgery, Geneva University Hospitals, University of Geneva, 1205 Geneva, Switzerland.
Chiraz ChaabaneGenomic Research Laboratory, Division of Infectious Diseases, Department of Medicine, Geneva University Hospitals, University of Geneva, 1205 Geneva, Switzerland.
Jacques SchrenzelGenomic Research Laboratory, Division of Infectious Diseases, Department of Medicine, Geneva University Hospitals, University of Geneva, 1205 Geneva, Switzerland.ORCID 0000-0001-5464-7764
Julian Grégoire LeprinceDivision of Cariology and Endodontology, University Clinics of Dental Medicine (CUMD), University of Geneva, 1211 Geneva, Switzerland.ORCID 0000-0001-7396-0019
Serge BouillaguetDivision of Cariology and Endodontology, University Clinics of Dental Medicine (CUMD), University of Geneva, 1211 Geneva, Switzerland.
Vladimir LazarevicGenomic Research Laboratory, Division of Infectious Diseases, Department of Medicine, Geneva University Hospitals, University of Geneva, 1205 Geneva, Switzerland.ORCID 0000-0002-6839-2536

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aims to contribute to the understanding of the dental pulp microbiome through metataxonomic analysis of pulp tissues from clinically healthy impacted third molars in 17 patients. Samples from coronal pulp, outer radicular dentin, and negative controls were included. Each sample was spiked with a known bacterial standard. Sequencing of the 16S rRNA V3-V4 region revealed similarity in bacterial taxonomic profiles. The bacterial DNA was detected in all pulp samples, primarily originating from reagent contaminants. Although some oral taxa appeared more abundant in pulp than in dentin or controls, contaminant species overwhelmingly dominated. Most of the extracted DNA was of human origin, rather than bacterial. Our findings question the concept of a core microbiome in healthy pulp and highlight the critical need for rigorous controls in pulpal microbiome studies.

Indexed as

BacteriaDental PulpMicrobiotaAdolescentAdultDNA, BacterialFemaleHumansMaleRNA, Ribosomal, 16SYoung AdultDNA, BacterialRNA, Ribosomal, 16S16S ribosomal RNAdental pulpmetataxonomicsmicrobiotanext-generation sequencingqPCR quantificationspike-in controls

Identifiers

PMID40244028
PMCPMC11989987

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.