Evidence map›Paper›PMID 40243586›Full record

ArticleInternational journal of molecular sciences2025

Alterations in Gene Expression and Alternative Splicing Induced by Plasmid-Mediated Overexpression of GFP and

Qingqing Liu, Zhaoyu Liu, Yongqi Qian, Mingxu Wu, Jing Mo, Can Wang, Guoqing Xu, Liang Leng, Sanyin Zhang

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Qingqing LiuCollege of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Zhaoyu LiuInstitute of Herbgenomics, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Yongqi QianCollege of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Mingxu WuCollege of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Jing MoInstitute of Herbgenomics, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Can WangInstitute of Herbgenomics, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Guoqing XuInstitute of Herbgenomics, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Liang LengInstitute of Herbgenomics, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.ORCID 0000-0002-9629-6808
Sanyin ZhangInnovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Phenotypic modifications and their effects on cellular functions through the up-regulation of target gene expression have frequently been observed in genetic studies, but the unique roles of cell lines and their introduced plasmids in influencing these functions have not been fully revealed. In this research, we developed two distinct cell lines derived from the A549 cell line: one that stably overexpresses GFP and another that is a polyclonal stable line overexpressing both GFP and

Indexed as

Alternative SplicingGene Expression Regulation, NeoplasticGreen Fluorescent ProteinsPlasmidsReceptors, Purinergic P2Y12A549 CellsCell MovementCell ProliferationGene Expression ProfilingHumansTranscriptomeGreen Fluorescent ProteinsReceptors, Purinergic P2Y12A549DEGsgDTUsGFPoverexpression cell lineP2RY12

Identifiers

PMID40243586
PMCPMC11988474

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.