Evidence map›Paper›PMID 40243479›Full record

ArticleInternational journal of molecular sciences2025

Protocols for Extraction of miRNA from Extracellular Vesicles of Lyophilized Human Saliva Samples.

Valquiria Quinelato, Carlos Fernando Mourão, Thalita Alves Barreto Santos, Patrícia Cataldo de Felipe Cordeiro, Leticia Ladeira Bonato, Miria Gomes Pereira, Jose Albuquerque Calasans-Maia, Jose Mauro Granjeiro, Tomoyuki Kawase, Priscila Ladeira Casado

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Valquiria QuinelatoPost-Graduation Program in Dentistry, Universidade Federal Fluminense, Niteroi 24020-140, Rio de Janeiro, Brazil.
Carlos Fernando MourãoDepartment of Basic and Clinical Translational Sciences, School of Dental Medicine, Tufts University, Boston, MA 02111, USA.ORCID 0000-0001-5775-0222
Thalita Alves Barreto SantosPost-Graduation Program in Dentistry, Universidade Federal Fluminense, Niteroi 24020-140, Rio de Janeiro, Brazil.
Patrícia Cataldo de Felipe CordeiroPost-Graduation Program in Dentistry, Universidade Federal Fluminense, Niteroi 24020-140, Rio de Janeiro, Brazil.
Leticia Ladeira BonatoPost-Graduation Program in Dentistry, Universidade Federal Fluminense, Niteroi 24020-140, Rio de Janeiro, Brazil.
Miria Gomes PereiraLaboratory of Cellular Ultrastructure Hertha Meyer, Biophysics Institute Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 1941-902, Rio de Janeiro, Brazil.ORCID 0000-0001-9103-0448
Jose Albuquerque Calasans-MaiaPost-Graduation Program in Dentistry, Universidade Federal Fluminense, Niteroi 24020-140, Rio de Janeiro, Brazil.ORCID 0000-0002-1498-726X
Jose Mauro GranjeiroPost-Graduation Program in Dentistry, Universidade Federal Fluminense, Niteroi 24020-140, Rio de Janeiro, Brazil.ORCID 0000-0002-8027-8293
Tomoyuki KawaseDivision of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata 950-8680, Japan.ORCID 0000-0003-4160-396X
Priscila Ladeira CasadoPost-Graduation Program in Dentistry, Universidade Federal Fluminense, Niteroi 24020-140, Rio de Janeiro, Brazil.

Funding

Japan Society for the Promotion of Science 24K14476
6 · The paper itself

Abstract

Extracellular vesicles (EVs) are emerging as crucial biomarkers in molecular diagnostics, providing early detection of disease progression. Although ultracentrifugation remains the gold standard for vesicle isolation from biofluids, it has limitations in scalability and accessibility. This study presents lyophilization as an innovative method for preserving EVs and isolating microRNAs from saliva, utilizing its proven ability to maintain biological activity and prevent unwanted chemical reactions. We assessed five different sample preparation protocols combined with a dual-purification strategy. Structural and molecular integrity analyses revealed that lyophilized samples retained essential EV characteristics, including CD63/CD9 membrane localization. QELS analysis and electron microscopy confirmed distinct vesicle populations in both ultracentrifuged (30-50 nm and 320-360 nm) and lyophilized samples (50-70 nm and 360-380 nm). Importantly, lyophilized samples exhibited higher total RNA concentrations (

Indexed as

Extracellular VesiclesMicroRNAsSalivaBiomarkersFreeze DryingHumansTetraspanin 30UltracentrifugationBiomarkersMicroRNAsTetraspanin 30exosomesextracellular vesicleslyophilizationmicroRNA

Identifiers

PMID40243479
PMCPMC11988657

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.