Evidence map›Paper›PMID 40243319›Full record

ArticleApplied and environmental microbiology2025

Characterization of 2-phenanthroyl-CoA reductase, an ATP-independent type III aryl-CoA reductase involved in anaerobic phenanthrene degradation.

Nadia A Samak, Frederik Götz, Khadija Adjir, Torsten Schaller, Marvin Häßler, Oliver J Schmitz, Jonas Fax, Gebhard Haberhauer, Alina Surmeneva, Rainer U Meckenstock

Abstract read
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In one paragraph

Article in Applied and environmental microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Nadia A SamakEnvironmental Microbiology and Biotechnology (EMB), Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany.ORCID 0000-0001-6575-2965
Frederik GötzEnvironmental Microbiology and Biotechnology (EMB), Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany.
Khadija AdjirLaboratory of Thermodynamics and Molecular Modeling, Faculty of Chemistry, USTHB, Algiers, Algeria.
Torsten SchallerOrganic Chemistry, Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany.
Marvin HäßlerApplied Analytical Chemistry, Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany.
Oliver J SchmitzApplied Analytical Chemistry, Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany.
Jonas FaxOrganic Chemistry, Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany.
Gebhard HaberhauerOrganic Chemistry, Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany.
Alina SurmenevaEnvironmental Microbiology and Biotechnology (EMB), Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany.
Rainer U MeckenstockEnvironmental Microbiology and Biotechnology (EMB), Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany.ORCID 0000-0001-7786-9546

Funding

Alexander von Humboldt-Stiftung
6 · The paper itself

Abstract

Anaerobic degradation of polycyclic aromatic hydrocarbons (PAHs) with three or more aromatic rings is extremely slow because the compounds are very poorly soluble in water and chemically stable. Phenanthrene is the only three-ring PAH where the anaerobic degradation has been partially elucidated. Phenanthrene is first activated via carboxylation producing 2-phenanthroate, which is further converted to 2-phenanthroyl-coenzyme A (CoA) via the enzyme 2-phenanthroate:CoA ligase. In this study, we elucidated the next degradation step, the reduction of 2-phenanthroyl-CoA to dihydro-2-phenanthroyl-CoA. We cloned the putative gene from the genome of culture TRIP_1 and heterologously expressed and purified the 2-phenanthroyl-CoA reductase enzyme from

Indexed as

PhenanthrenesAcyl Coenzyme AAnaerobiosisBiodegradation, EnvironmentalCloning, MolecularEscherichia coliAcyl Coenzyme AphenanthrenePhenanthrenes2-phenanthroyl-CoAanaerobic phenanthrene degradationculture TRIP_1old-yellow enzymePAHsreductase

Identifiers

PMID40243319
PMCPMC12093976

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.