Evidence map›Paper›PMID 40243305›Full record

ArticleMicrobiology spectrum2025

Markerless mutagenesis enables isoleucine biosynthesis solely from threonine in

Maximilian Klein, Angus S Hilts, Ross T Fennessy, Nino Trattnig, Thomas Stehrer-Polášek, Simon K-M R Rittmann, Christian Fink

Abstract read
In one paragraph

Article in Microbiology spectrum, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Metabolic engineering ofMetabolic engineering communications · 2026
    Article
  2. Valine overproduction inMetabolic engineering communications · 2026
    Article
  3. Article
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Maximilian KleinArkeon GmbH, Tulln a.d. Donau, Austria.
Angus S HiltsArkeon GmbH, Tulln a.d. Donau, Austria.
Ross T FennessyArkeon GmbH, Tulln a.d. Donau, Austria.
Nino TrattnigArkeon GmbH, Tulln a.d. Donau, Austria.
Thomas Stehrer-PolášekArkeon GmbH, Tulln a.d. Donau, Austria.
Simon K-M R RittmannArkeon GmbH, Tulln a.d. Donau, Austria.ORCID 0000-0002-9746-3284
Christian FinkArkeon GmbH, Tulln a.d. Donau, Austria.ORCID 0000-0003-4233-014X

Funding

Austrian Centre of Industrial BiotechnologyÖsterreichische Forschungsförderungsgesellschaft FO999901140
6 · The paper itself

Abstract

The archaeal model microorganism IMPORTANCE: The autotrophic, hydrogenotrophic methanogen

Indexed as

IsoleucineMethanobacteriaceaeMutagenesisThreonineArchaeal ProteinsBiosynthetic PathwaysMetabolic EngineeringPyruvic AcidArchaeal ProteinsIsoleucinePyruvic AcidThreonineamino acid productionarchaeabiotechnologymetabolic engineeringmethanogenssynthetic biology

Identifiers

PMID40243305
PMCPMC12131731

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.