Evidence map›Paper›PMID 40241136›Full record

ArticleOne health outlook2025

Extended spectrum beta-lactamase producing Escherichia coli and antimicrobial resistance gene sharing at the interface of human, poultry and environment: results of ESBL tricycle surveillance in Kathmandu, Nepal.

Jyoti Acharya, Runa Jha, Ranjan Raj Bhatta, Lilee Shrestha, Barun Kumar Sharma, Sharmila Chapagain, Tulsi Ram Gompo, Nisha Rijal, Priya Jha, Sarah L Baines and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in One health outlook, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Slaughterhouse Wastewater as a Reservoir of ThermotolerantInternational journal of microbiology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Jyoti AcharyaNational Public Health Laboratory, Teku, Kathmandu, Nepal.
Runa JhaNational Public Health Laboratory, Teku, Kathmandu, Nepal.
Ranjan Raj BhattaNational Public Health Laboratory, Teku, Kathmandu, Nepal.
Lilee ShresthaNational Public Health Laboratory, Teku, Kathmandu, Nepal.
Barun Kumar SharmaCentral Veterinary Laboratory, Tripureshwor, Kathmandu, Nepal.
Sharmila ChapagainCentral Veterinary Laboratory, Tripureshwor, Kathmandu, Nepal.
Tulsi Ram GompoCentral Veterinary Laboratory, Tripureshwor, Kathmandu, Nepal.
Nisha RijalWHO Country Office, Nepal, UN House, Kupondole, Pulchowk, Lalitpur, Kathmandu, Nepal.
Priya JhaWHO Country Office, Nepal, UN House, Kupondole, Pulchowk, Lalitpur, Kathmandu, Nepal.
Sarah L Baines *Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
Louise M JuddDepartment of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
Lisa IoannidisDepartment of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
Benjamin P Howden *Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
Palpasa KansakarWHO Country Office, Nepal, UN House, Kupondole, Pulchowk, Lalitpur, Kathmandu, Nepal. pkansakar@who.int.

Funding

World Health Organization 001
6 · The paper itself

Abstract

backgroundThe spread of antimicrobial resistant pathogens, including extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae is a global health threat and can be addressed only through a One Health approach. We aimed to characterize ESBL producing Escherichia coli isolates from World Health Organization Tricycle surveillance using data from whole genome sequencing (WGS) to decipher the potential dynamics of their circulation at the human, poultry and environment interface.

methodsWGS was performed on 100 non-duplicate representative ESBL E. coli isolates including 28 isolates from humans, 36 from poultry caeca, and 36 from water samples. Minimum Inhibitory Concentration (MIC) was determined using Vitek 2 Compact. WGS was performed on Illumina NextSeq 2000 platform and open-source bioinformatics pipelines were used to analyze WGS data for genomic characterization including phylogenetic analysis and in silico multi-locus sequence typing and, serotyping and, ESBL gene detection.

resultsMost isolates were susceptible to imipenem (98%), meropenem (94%) and tigecycline (94%). Six ESBL E. coli isolates from poultry were resistant to colistin (MIC ≥ 4 μg/ml). WGS revealed high genetic diversity representing 56 sequence types (ST) including three novel STs. ST131 (7 isolates) was the most prevalent comprising human and environment isolates, followed by ST2179 (6 isolates, all poultry) and ST155 (5 isolates across the three sectors). All eight recognized E. coli phylogroups were observed, with majority (86%) of the isolates belonging to A, B1, B2 and D phylogroups. Of the100 isolates, 98 carried bla

conclusionOur findings indicate high genetic diversity among ESBL E. coli strains from all three sectors and sharing of identical strains and resistance genes within and between sectors. ST131, the globally dominant ESBL E. coli clade is gaining prevalence in Nepal with bla

Indexed as

bla CTX−MESBL E. coliNepalOne health

Identifiers

PMID40241136
PMCPMC12004597

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.