ArticleJournal of biological engineering2025
Unraveling the unique bioactivities of highly purified C-phycocyanin and allophycocyanin.
Article in Journal of biological engineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
3 citing papers in PubMed.
- Red Seaweed-Derived Phycobiliproteins: Marine Bioactive Colorants with Functional Health Properties.Marine drugs · 2026Review
- Neuroprotective effects of C-phycocyanin and allophycocyanin from Spirulina purified using a newly developed method: in vitro barrier permeability, antioxidant and anti-inflammatory activities.Journal of biological engineering · 2026Article
- Spirulina and Its Bioactive Compounds as Multi-Target Anticancer Agents: Mechanisms, Immune Modulation, and Translational Potential.Medical sciences (Basel, Switzerland) · 2026Review
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Authors and funding
8 authors.
Funding
Abstract
backgroundThe blue-green microalgae Spirulina, used in human nutrition for centuries, includes phycobiliproteins such as C-phycocyanin (CPC) and allophycocyanin (APC). Assessing their unique bioactivities separately is difficult as they have similar properties, such as molecular weight and isoelectric point. In the present study, we aimed to separate CPC and APC and to evaluate their bioactivities. CPC and APC were separated using a hydrophobic membrane and ammonium sulfate, which promotes reversible and specific protein binding to the membrane. Spectroscopic analysis, HPLC, and SDS-PAGE revealed a successful separation of CPC and APC. Their bioactivities were evaluated through CCK- 8 assays for anticancer activity, radical scavenging assays for antioxidant activity, and albumin denaturation assays for anti-inflammatory activity, respectively.
resultsThe results revealed that highly purified APC showed 40% higher anticancer activity than the control, whereas CPC increased the viability of cancer cells, resulting in a 30% decrease in anticancer activity compared to the control. In contrast, highly purified CPC showed approximately 25% higher antioxidant activity and twice as much anti-inflammatory activity as APCs; moreover, the presence of both showed higher antioxidant activity.
conclusionThis study provides important insights into the unique bioactivities of CPC and APC for their appropriate application as anticancer, antiphlogistic, and antioxidant agents.
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