Evidence map›Paper›PMID 40240940›Full record

ArticleBMC infectious diseases2025

Development of a PCR-dipstick DNA chromatography-based tool for the detection of CTX-M- and TEM-producing Escherichia coli and Klebsiella pneumoniae isolated from patients in Kafue and Katete districts of Zambia.

Misheck Shawa, Kyoko Hayashida, Naganori Nao, Atmika Paudel, Harvey Kamboyi, Herman Chambaro, Malala Mulavu, Cynthia Sipho Khumalo, Mike Nundwe, Tuvshinzaya Zorigt and 18 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC infectious diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Frontiers in microbiology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

28 authors.

Misheck ShawaHokudai Center for Zoonosis Control in Zambia, Hokkaido University, Lusaka, 10101, Zambia. misheckshawa@czc.hokudai.ac.jp.
Kyoko HayashidaHokudai Center for Zoonosis Control in Zambia, Hokkaido University, Lusaka, 10101, Zambia.
Naganori NaoHokudai Center for Zoonosis Control in Zambia, Hokkaido University, Lusaka, 10101, Zambia.
Atmika PaudelGenEndeavor LLC, 26219 Eden Landing Rd, Hayward, CA, 94545, USA.
Harvey KamboyiDivision of Infection and Immunity, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan.
Herman ChambaroCentral Veterinary Research Institute, Ministry of Fisheries and Livestock, Lusaka, 10101, Zambia.
Malala MulavuDepartment of Biomedical Sciences, School of Health Sciences, University of Zambia, Lusaka, 10101, Zambia.
Cynthia Sipho KhumaloDepartment of Biological Sciences, School of Veterinary Medicine, University of Zambia, Lusaka, 10101, Zambia.
Mike NundweInstitute of Basic and Biomedical Sciences, Levy Mwanawasa Medical University, 10101, Lusaka, Zambia.
Tuvshinzaya ZorigtDivision of Infection and Immunity, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan.
Chinatsu NakamuraHokudai Center for Zoonosis Control in Zambia, Hokkaido University, Lusaka, 10101, Zambia.
Yongjin QiuHokudai Center for Zoonosis Control in Zambia, Hokkaido University, Lusaka, 10101, Zambia.
Naoko KawaiDivision of Collaboration and Education, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan.
Maisa KasangaDepartment of Pathology and Microbiology, University Teaching Hospital, Lusaka, 10101, Zambia.
Joseph ChizimuZambia National Public Health Institute, Ministry of Health, Lusaka, 10101, Zambia.
Lavel MoongaDepartment of Para-Clinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka, 10101, Zambia.
Joseph NdebeDepartment of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, 10101, Zambia.
Manyando SimbotweDepartment of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, 10101, Zambia.
Shohei OgataHokudai Center for Zoonosis Control in Zambia, Hokkaido University, Lusaka, 10101, Zambia.
Mulemba SamutelaDepartment of Biomedical Sciences, School of Health Sciences, University of Zambia, Lusaka, 10101, Zambia.
Chie NakajimaDivision of Bioresources, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan.
Roma ChilengiZambia National Public Health Institute, Ministry of Health, Lusaka, 10101, Zambia.
Mable MutengoInstitute of Basic and Biomedical Sciences, Levy Mwanawasa Medical University, 10101, Lusaka, Zambia.
Masahiro KajiharaHokudai Center for Zoonosis Control in Zambia, Hokkaido University, Lusaka, 10101, Zambia.
Hirofumi SawaHokudai Center for Zoonosis Control in Zambia, Hokkaido University, Lusaka, 10101, Zambia.
Bernard Hang'ombeDepartment of Para-Clinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka, 10101, Zambia.
Yasuhiko SuzukiDivision of Bioresources, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan.
Hideaki HigashiDivision of Infection and Immunity, International Institute for Zoonosis Control, Hokkaido University, N20 W10, Kita-Ku, Sapporo, 001-0020, Japan.

Funding

Japan Agency for Medical Research and Development JP23wm0125008Japan Society for the Promotion of Science 21K15430Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan, for the Joint Research Program of the Hokkaido University International Institute for Zoonosis Control JP223fa627005
6 · The paper itself

Abstract

In Zambia, 40% of clinical Gram-negative bacteria are either Escherichia coli or Klebsiella pneumoniae, with a high third-generation cephalosporin (3GC) resistance prevalence. Therefore, 3GC resistance surveillance is a crucial indicator for guiding focused intervention policies. However, the lack of genotypic diagnostic tools limits the ability to elucidate trends, especially in peri-urban and rural areas of developing countries. This study aimed to develop a rapid, cost-effective tool for the genotypic surveillance of 3GC resistance. Here, 900 stool samples collected from patients in Kafue (peri-urban, n = 400) and Katete (rural, n = 500) districts of Zambia were used for bacterial isolation on MacConkey agar supplemented with 1 μg/ml cefotaxime. Isolated 3GC-resistant strains were characterized by sequencing the 16S rRNA gene and screening for bla

Indexed as

beta-LactamasesEscherichia coliEscherichia coli InfectionsEscherichia coli ProteinsKlebsiella InfectionsKlebsiella pneumoniaePolymerase Chain ReactionAnti-Bacterial AgentsDrug Resistance, BacterialFecesHumansMicrobial Sensitivity TestsWhole Genome SequencingZambiaAnti-Bacterial Agentsbeta-LactamasesEscherichia coli Proteins3GCBla CTX-MBla TEME. coliK. pneumoniaeSTH-PASZambia

Identifiers

PMID40240940
PMCPMC12004789

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.