Evidence map›Paper›PMID 40240791›Full record

ArticleScientific reports2025

HOXD1 regulates neural crest cells differentiation and polycerate development in sheep.

Cheng Zhang, Shuhong Zhang, Huifeng Xu, Guan Wang, Huan Zhang, Tenggang Di, Liming Tian, Menghan Chang, Fengyi Gao, Ming Li and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Cheng ZhangCollege of Biology and Food, Shangqiu Normal University, Shangqiu, 476000, China.
Shuhong ZhangCollege of Biology and Food, Shangqiu Normal University, Shangqiu, 476000, China.
Huifeng XuCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450002, China.
Guan WangCollege of Biology and Food, Shangqiu Normal University, Shangqiu, 476000, China.
Huan ZhangCollege of Biology and Food, Shangqiu Normal University, Shangqiu, 476000, China.
Tenggang DiCollege of Biology and Food, Shangqiu Normal University, Shangqiu, 476000, China.
Liming TianCollege of Biology and Food, Shangqiu Normal University, Shangqiu, 476000, China.
Menghan ChangCollege of Biology and Food, Shangqiu Normal University, Shangqiu, 476000, China.
Fengyi GaoCollege of Biology and Food, Shangqiu Normal University, Shangqiu, 476000, China.
Ming LiCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450002, China. liming@henau.edu.cn.
Guangli YangCollege of Biology and Food, Shangqiu Normal University, Shangqiu, 476000, China. guangliyang@163.com.

Funding

Promotion Program of He'nan Science and Technology Committee of China 252102110076Research Program of Foundation and Advanced Technology of He'nan Science and Technology Committee of China 102300410143, 132300410398The Foundation of He'nan Educational Committee of China 25A230006
6 · The paper itself

Abstract

The polycerate trait in sheep is a complex phenotype regulated by polygenes. However, the mechanism behind multi-horned traits development and growth remains unclear. In this study, neural crest cells (NCCs) were isolated from mouse embryos, and the HOXD1 (Homeobox D1) gene was overexpressed in these cells to identify its function. Transcriptome analysis was performed to explore the key signaling pathway involved in forming the multi-horned traits in sheep. The results showed that the HOXD1 induced epithelial-to-mesenchymal transition (EMT) in mouse neural crest primary cells, affecting their migration but without significantly influencing proliferation. Furthermore, signaling pathway analysis suggested that HOXD1 may inhibit NCC proliferation by modulating Wnt rather than TGF-β signaling. Transcriptome analysis revealed that the HOXD1 gene affected the extracellular matrix of CXC family regulatory cells and promoted NCC differentiation. These findings provide a theoretical basis for further investigation into the regulation of multi-horned traits growth and development in sheep.

Indexed as

Cell DifferentiationHomeodomain ProteinsNeural CrestTranscription FactorsAnimalsCell MovementCell ProliferationEpithelial-Mesenchymal TransitionGene Expression ProfilingGene Expression Regulation, DevelopmentalMiceSheepWnt Signaling PathwayHomeodomain ProteinsTranscription FactorsChemokine CXCHOXD1 geneNeural crest cellsPolycerate traitSheep

Identifiers

PMID40240791
PMCPMC12003793

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.