Evidence map›Paper›PMID 40240732›Full record

ArticleJournal of the Association for Research in Otolaryngology : JARO2025

Influence of Myosin Regulatory Light Chain and Myosin Light Chain Kinase on the Physiological Function of Inner Ear Hair Cells.

Ryohei Oya, Kwang Min Woo, Brian Fabella, R G Alonso, Paloma Bravo, A J Hudspeth

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Article in Journal of the Association for Research in Otolaryngology : JARO, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in
1citing papers in PubMed
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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

6 authors.

Ryohei OyaHoward Hughes Medical Institute, The Rockefeller University, New York, NY, 10065, USA. roya@ent.med.osaka-u.ac.jp.ORCID http://orcid.org/0000-0001-8991-0315
Kwang Min WooLaboratory of Sensory Neuroscience, The Rockefeller University, 1230 York Avenue, New York, NY, 10065, USA.
Brian FabellaHoward Hughes Medical Institute, The Rockefeller University, New York, NY, 10065, USA.
R G AlonsoHoward Hughes Medical Institute, The Rockefeller University, New York, NY, 10065, USA.
Paloma BravoLaboratory of Sensory Neuroscience, The Rockefeller University, 1230 York Avenue, New York, NY, 10065, USA.
A J HudspethHoward Hughes Medical Institute, The Rockefeller University, New York, NY, 10065, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeIn the receptor organs of the inner ear, hair cells detect mechanical stimuli such as sounds and accelerations by deflection of their hair bundles. Myosin regulatory light chain (RLC) and non-muscle myosin II (NM2) are expressed at the apical surfaces of hair cells, and NM2 and the phosphorylation of RLC by myosin light chain kinase (MLCK) have earlier been shown to regulate the shapes of hair cells' apical surfaces in rodents. The aim of our study was to elucidate the function of myosin molecules on hair cell physiology.

methodsWe investigated the expression of NM2 and RLC in the bullfrog's saccule by immunostaining. Using NM2 and MLCK inhibitors, we measured the stiffness, spontaneous oscillation, and resting open probability of frog hair bundles. Six to ten saccules from pleural animals were used in each experiment. In addition, we recorded auditory brainstem responses in ten mice after transtympanic injection of an MLCK inhibitor.

resultsWe confirmed the expression of NM2A/B and MYL9 on the apical surfaces of hair cells and of NM2A and MYL12A in hair bundles. We found that NM2 and MLCK inhibitors reduce the stiffness of hair bundles from the bullfrog's saccule. Moreover, MLCK inhibition inhibits the spontaneous oscillation of hair bundles and increases the resting open probability of transduction channels. In addition, MLCK inhibition elevates hearing thresholds in mice.

conclusionWe conclude that NM2 and the phosphorylation of RLC modulate the physiological function of hair cells and thereby help to set the normal operating conditions of hair bundles.

Indexed as

Hair Cells, Auditory, InnerMyosin-Light-Chain KinaseMyosin Light ChainsAnimalsEvoked Potentials, Auditory, Brain StemMaleMiceMyosin Type IIPhosphorylationRana catesbeianaSaccule and UtricleMyosin-Light-Chain KinaseMyosin Light ChainsMyosin Type IIActive processAuditory systemCochleaSpontaneous oscillationVestibular system

Identifiers

PMID40240732
PMCPMC12133660

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.