ArticleScience advances2025
A palette of bright and photostable monomeric fluorescent proteins for bacterial time-lapse imaging.
Article in Science advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Serendipitous crystal structure of mJuniper, a rationally designed cyan fluorescent protein.Acta crystallographica. Section F, Structural biology communications · 2026Article
- Constitutive, endogenous, fluorescent membrane reporters for dynamic cell cycle analysis inApplied and environmental microbiology · 2026Article
- Mechanism of persister formation in response to nitrogen starvation.Nature communications · 2026Article
- Expanding salivary biomarker detection by creating a synthetic neuraminic acid sensor via chimeragenesis.Applied and environmental microbiology · 2026Article
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- Single-cell phenotypic heterogeneity shapes quorum signaling dynamics in Pseudomonas aeruginosa.Nature communications · 2026Article
- Chemical biology approaches to study and target circadian clocks and their components.FEBS letters · 2026Review
- mSumireF a Monomeric Violet Fluorescent Protein.ACS bio & med chem Au · 2026Article
- Tags Attached to the Centrosomal Protein SPD-5 Can Disrupt Centrosome Flattening During Anaphase of the 1-cellmicroPublication biology · 2026Article
- Phenylacetic acid mediatesMicrobiology (Reading, England) · 2026Article
- Control of flagellar gene expression by a chemotaxis receptor-like regulator in pathogenic Escherichia coli.The EMBO journal · 2025Article
- Article
- A blueprint for biomolecular condensation driven by bacterial microcompartment encapsulation peptides.Nature communications · 2025Article
- Conjugative delivery of toxin genesJournal of bacteriology · 2025Article
- Robust Synthetic Biology Toolkit to Advance Carboxysome Study and Redesign.ACS synthetic biology · 2025Article
- Tissue-like multicellular development triggered by mechanical compression in archaea.Science (New York, N.Y.) · 2025Article
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Authors and funding
4 authors.
Funding
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Abstract
Fluorescent proteins (FPs) are pivotal for examining protein production, localization, and dynamics in live bacterial cells. However, the use of FPs in time-lapse imaging is frequently constrained by issues such as oligomerization or limited photostability. Here, we report the engineering of novel cyan, green, yellow, and red FPs that exhibit improved photostability and aggregation properties while retaining high in vivo brightness. We first derived superfolder green fluorescent protein into mChartreuse, a brighter, more photostable, and monomeric fluorophore. mChartreuse was further derived into cyan and yellow variants with enhanced photostability and dispersibility. We also report a mutation that eliminates residual oligomerization in red FPs derived from
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