Evidence map›Paper›PMID 40237440›Full record

ArticleCNS neuroscience & therapeutics2025

MK5 Regulates Microglial Activation and Neuroinflammation in Experimental Stroke Models.

Xingzhi Wang, Wenqi Mao, Li Du, Fei Wang, Ye Pang, Yangdanyu Li, Guangci Xu, Guiyun Cui

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Article in CNS neuroscience & therapeutics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Xingzhi WangDepartment of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.ORCID 0000-0001-9248-3510
Wenqi MaoDepartment of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Li DuDepartment of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Fei WangDepartment of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Ye PangDepartment of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Yangdanyu LiDepartment of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Guangci XuDepartment of Neurology, The First People's Hospital of Sihong Count, Suqian, Jiangsu, China.
Guiyun CuiDepartment of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.ORCID 0000-0002-8467-5798

Funding

Leadership program through open competition in Xuzhou Medical University JBGS202203National Natural Science Foundation of China 82171305Paired Assistance Scientific Research Project by The Affiliated Hospital of Xuzhou Medical University SHJDBF2024103Postgraduate Research & Practice Innovation Program of Jiangsu Province SJCX24_1569the Open Project of Key Laboratory of Colleges and Universities in Jiangsu Province XZSYSKF2022021The Science and Technology Planning Project of Xuzhou KC23267the Science and Technology Project of Xuzhou Health Commission XWKYHT20220156
6 · The paper itself

Abstract

objectiveMicroglial activation plays a crucial role in neuroinflammation following ischemic stroke. This study was conducted to investigate the role and potential mechanisms of MK5 within microglial cells in the inflammatory response following ischemic stroke in mice in vivo and in vitro.

methodsMicroglia-specific conditional MK5 knockout (MK5 cKO) mice and their control mice (MK5

resultsCompared to the control group, the knockout of the MK5 gene in microglia significantly exacerbated neurological deficits and increased infarct volume in MCAO mice. The loss of the MK5 promoted inflammation by upregulating pro-inflammatory factors and downregulating anti-inflammatory factors, while also enhancing microglial activation in both MCAO mice and BV2 microglial cells subjected to OGD/R. Furthermore, the knockout of the MK5 gene in microglia reduced the phosphorylation levels of HSP27 and increased the phosphorylation levels of NF-κB in the aforementioned models.

conclusionMicroglial MK5 plays a critical role in the ischemic neuroinflammatory response by regulating the phosphorylation of HSP27 and NF-κB, positioning it as a potential target for stroke treatment.

Indexed as

Infarction, Middle Cerebral ArteryIntracellular Signaling Peptides and ProteinsMicrogliaNeuroinflammatory DiseasesProtein Serine-Threonine KinasesStrokeAnimalsDisease Models, AnimalMaleMiceMice, Inbred C57BLMice, KnockoutIntracellular Signaling Peptides and ProteinsProtein Serine-Threonine KinasesHSP27ischemic strokemicrogliaMK5neuroinflammation

Identifiers

PMID40237440
PMCPMC12001269

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.