ArticleFrontiers in cellular and infection microbiology2025
Development and application of a dual LAMP-LFD assay for the simultaneous detection of
Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Development of a loop-mediated isothermal amplification-lateral flow dipstick (LAMP-LFD) assay and on-site rapid detection evaluation for Rodentibacter heylii and Rodentibacter pneumotropicus.Animal models and experimental medicine · 2026Article
- Epidemiology of Major Bacterial Pathogens Associated with Porcine Respiratory Disease Complex: A Cross-Sectional Study from Intensive Swine Farms in Xinjiang, China (2024-2025).Veterinary sciences · 2026Article
- Rapid visual detection ofOpen veterinary journal · 2026Article
- Rapid and specific detection ofFrontiers in veterinary science · 2025Article
Corrections and comments
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Authors and funding
7 authors.
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Abstract
Introduction: Methods: The assay utilized primers targeting the conserved regions of the gdh gene of S. suis and the infB gene of Results: The assay was most effective at a reaction temperature of 62°C, a primer concentration ratio of 1:4, and a reaction time of 40 minutes. The minimum detection limits were 22 and 18 copies/μL for recombinant plasmids and 19 and 20 CFU for bacterial samples of S. suis and G. parasuis, respectively. The assay showed no cross-reactivity with other pathogens and exhibited high adaptability across various thermal platforms, including PCR instruments, metal baths, and water baths. Clinical testing of 106 samples revealed positive rates of 11.32% (12/106) for S. suis, 25.47% (27/106) for Discussion: This simple, rapid, specific, and sensitive dual LAMP-LFD assay provides robust technical support for the prevention and control of swine streptococcosis and Glässer's disease.
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