Evidence map›Paper›PMID 40235914›Full record

ArticleFrontiers in plant science2025

Effects of phospholipase C inhibition on the regulation of membrane lipid metabolism in maize leaves.

Yulei Wei, Zhicheng Cai, Zhiyuan Che, Xu Guo, Shengnan Ge, Xinyang Che, Jie Deng, Haiyang Zhang, Lin He, Jingyu Xu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in plant science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yulei WeiKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, China.
Zhicheng CaiKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, China.
Zhiyuan CheKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, China.
Xu GuoKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, China.
Shengnan GeKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, China.
Xinyang CheKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, China.
Jie DengKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, China.
Haiyang ZhangKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, China.
Lin HeKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, China.
Jingyu XuKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Phospholipase C (PLC) is an enzyme that catalyzes the hydrolysis of glycerophospholipids and can be classified as phospholipase-specific PLC (PI-PLC) and non-specific PLC (NPC) depending on its specific substrate. Methods: In this study, neomycin sulfate (NS, 100 mM) was used to inhibit the activity of phospholipase C in maize seedlings, and the effect of phospholipase C on lipid metabolism was investigated by combined analysis of transcriptome and lipidome. Results: Lipidomic analysis showed that when PLC was inhibited, the content of phospholipids showed more than 10% increase due to the elevated accumulation of PC and PE. At the same time, transcriptomic data suggested an activation of the de novo biosynthesis of PC and PE from choline and ethanolamine via upregulated CCT and PECT, respectively. Thus, the inhibition of phospholipid hydrolysis and the enhancement of de novo synthesis together contribute to the increase in the total phospholipids. Glycolipids account for around 60% of the total lipids in leaves. Under NS treatment, MGDG decreased significantly, while DGDG increased. Discussion: Our results indicate that NS treatment may cause damage to photosynthetic membrane lipids, whereas the increase in bilayer lipid DGDG may provide a kind of protection to maintain the stability of chloroplasts. These findings suggest that phospholipase C plays a key role in plasma membrane metabolism.

Indexed as

lipidomemaize (Zea mays L.)neomycin sulfatephospholipase Ctranscriptome

Identifiers

PMID40235914
PMCPMC11996868

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.