Genome-resolved metatranscriptomics unveils distinct microbial functionalities across aggregate sizes in aerobic granular sludge.

A Y A Mohamed, Laurence Gill, Alejandro Monleon, Mario Pronk, Mark van Loosdrecht, Pascal E Saikaly, Muhammad Ali

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Article in Environmental science and ecotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed

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1 citing paper in PubMed.

Sulfur-cycling diazotrophs dominate nitrogen fixation in seagrass sediments.Applied and environmental microbiology · 2026
Article

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5 · Who and what money

Authors and funding

7 authors.

A Y A MohamedDepartment of Civil, Structural & Environmental Engineering, Trinity College Dublin, The University of Dublin, Dublin 2, Ireland.

Laurence GillDepartment of Civil, Structural & Environmental Engineering, Trinity College Dublin, The University of Dublin, Dublin 2, Ireland.

Alejandro MonleonDepartment of Civil, Structural & Environmental Engineering, Trinity College Dublin, The University of Dublin, Dublin 2, Ireland.

Mario PronkDepartment of Biotechnology, Delft University of Technology, Delft, 2629 HZ, the Netherlands.

Mark van LoosdrechtDepartment of Biotechnology, Delft University of Technology, Delft, 2629 HZ, the Netherlands.

Pascal E SaikalyEnvironmental Science and Engineering Program, Biological and Environmental Science and Engineering (BESE) Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia.

Muhammad AliDepartment of Civil, Structural & Environmental Engineering, Trinity College Dublin, The University of Dublin, Dublin 2, Ireland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microbial aggregates of different sizes in aerobic granular sludge (AGS) systems have been shown to exhibit distinct microbial community compositions. However, studies comparing the microbial activities of different-sized aggregates in AGS systems remain limited. In this study, genome-resolved metatranscriptomics was used to investigate microbial activity patterns within differently sized aggregates in a full-scale AGS plant. Our analysis revealed a weak correlation between the relative abundance of metagenome-assembled genomes (MAGs) and their transcriptomic activity, indicating that microbial abundance does not directly correspond to metabolic activity within the system. Flocculent sludge (FL; <0.2 mm) predominantly featured active nitrifiers and fermentative polyphosphate-accumulating organisms (PAOs) from

Indexed as

Aerobic granular sludgeDifferential expression analysisGenome-resolved metatranscriptomicsMicrobial activityWastewater treatment

Identifiers

PMID40235649

DOI10.1016/j.ese.2025.100560

PMCPMC11999188

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