Evidence map›Paper›PMID 40234717›Full record

Observational studyPediatric research2025

Necrotizing enterocolitis: diagnosis with plasma IgG anti-tissue transglutaminase antibodies.

Hua Li, Chaoting Lan, Shuo Chen, Chun Yan, Shuchen Huangfu, Bowen Tian, Lin Li, Yide Mu, Shenwei Huang, Jiemei Liang and 4 more

Abstract readObservational Study
PubMed Publisher
In one paragraph

Observational study in Pediatric research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Hua Li *Department of Child Healthcare, Department of Pediatric Surgery, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.
Chaoting Lan *Department of Child Healthcare, Department of Pediatric Surgery, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.
Shuo Chen *Emergency Department, the 1st affiliated hospital, Jiangxi Medical College, Nanchang University, Nanchang, China.
Chun YanCenter for Medical Research on Innovation and Translation, Guangzhou First People's Hospital, Guangzhou, China.
Shuchen HuangfuCenter for Medical Research on Innovation and Translation, Guangzhou First People's Hospital, Guangzhou, China.
Bowen TianDepartment of Child Healthcare, Department of Pediatric Surgery, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.
Lin LiDepartment of Child Healthcare, Department of Pediatric Surgery, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.
Yide MuDepartment of Child Healthcare, Department of Pediatric Surgery, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.
Shenwei HuangDepartment of Child Healthcare, Department of Pediatric Surgery, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.
Jiemei LiangDepartment of Child Healthcare, Department of Pediatric Surgery, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.
Liqiong ZhuDepartment of Child Healthcare, Department of Pediatric Surgery, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.
Junjian LvDepartment of Child Healthcare, Department of Pediatric Surgery, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China. lyujunjian916@163.com.
Yufeng LiuCenter for Medical Research on Innovation and Translation, Guangzhou First People's Hospital, Guangzhou, China. eyyufengliu@scut.edu.cn.
Yan TianDepartment of Anesthesiology, Jiangxi Provincial Children's Hospital, Nanchang Medical College, Nanchang, Jiangxi, China. 18070504107@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundNecrotizing enterocolitis (NEC) is a severe inflammatory gastrointestinal disease in neonates. We aimed to evaluate the potential of IgG anti-tissue transglutaminase antibodies (IgG tTG) as early biomarkers for NEC.

methodWe conducted a prospective observational study, collecting plasma from 60 neonates with abdominal distension (AD), which were divided into the NEC (n = 30) and the controls (n = 30) according to the follow-up results, and used the autoantigen microarray to identify for NEC-associated autoantibodies. An Enzyme-linked immunosorbent assay (ELISA) was utilized to measure plasma IgG tTG levels in a validation study (NEC n = 43, controls n = 20).

resultsMicroarray analysis indicated significantly elevated IgG tTG levels in neonates with NEC compared to controls (P < 0.001). ELISA further confirmed the significant elevation of IgG tTG in neonates with NEC (P < 0.001). IgG tTG effectively distinguished neonates with NEC from the controls, with an area under the curve (AUC) of 0.8674 (sensitivity of 72.09% and specificity of 95%). Encouragingly, IgG tTG levels were significantly higher in neonates with NEC I than in controls (P < 0.001). Additionally, as clinical conditions of neonates with NEC improved, the IgG tTG levels declined (P = 0.0159).

conclusionIgG tTG has potential as a biomarker for early diagnosis of NEC and predicting its prognosis after treatment. IMPACT: Our results demonstrate that IgG anti-tissue transglutaminase antibodies possess significant diagnostic value for NEC. IgG anti-tissue transglutaminase antibodies can serve as a biomarker for early diagnosis NEC and its prognosis of treatment. The relationship between IgG anti-tissue transglutaminase antibodies and immune diseases may shed light on the pathogenesis of NEC, potentially opening new therapeutic avenues for preventing and treating neonates with NEC.

Indexed as

AutoantibodiesEnterocolitis, NecrotizingGTP-Binding ProteinsImmunoglobulin GTransglutaminasesBiomarkersCase-Control StudiesEnzyme-Linked Immunosorbent AssayFemaleHumansInfant, NewbornMaleProspective StudiesProtein Glutamine gamma Glutamyltransferase 2ROC CurveAutoantibodiesBiomarkersGTP-Binding ProteinsImmunoglobulin GProtein Glutamine gamma Glutamyltransferase 2Transglutaminases

Identifiers

PMID40234717

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.